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Abstract：
This protocol determines the fraction of a bacterial population that is viable and culturable, viable and non-culturable, or non-viable (dead). Each population is detected by isolating colonies on agar plates, performing direct counts, and staining for live or dead cells. Its application is limited to bacteria that are stainable and when permissible growth conditions are known. The quantitative data extracted allow for the detection of a viable but non-culturable (alive and non-dividing) population from a liquid culture. For complete details on the use and execution of this protocol, please refer to Stott et al. (2015).
摘要：
该协议确定了细菌种群的一部分是可行的和可培养的，可行且不可培养，或不可行（死亡）。通过在琼脂平板上分离菌落来检测每个种群，执行直接计数，对活细胞或死细胞进行染色。它的应用仅限于可染色的细菌，并且在已知允许的生长条件时。提取的定量数据允许检测来自液体培养物的活的但不可培养的(活的和非分裂的)群体。有关此协议的使用和执行的完整详细信息，请参考Stott等人。(2015)。
