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Abstract：
Triclocarban (TCC), an antimicrobial compound commonly added to a wide range of household and personal hygiene care products, is one of the most prevalent endocrine-disrupting substances (EDS). This study was conducted to elucidate whether in utero and lactational exposure to TCC could adversely affect folliculogenesis and the onset of puberty in female rat offspring. Twenty pregnant Sprague Dawley rats were equally divided into Control and TCC dam groups (supplemented daily with drinking water enriched with 0.5 mg/L of TCC) from gestational day5 to postnatal day21 (PND21). Female offspring, 20 from control and 20 from TCC dams, were subdivided into 4 subgroups (PND21, PND28, PND35 & PND42). The day of vaginal opening and first estrous cycle were determined. Ovarian sections of the offspring were processed for H&E staining and for immunohistochemical expression of Ki67, Caspase-3 and androgen receptors (AR) on the granulosa cells of ovarian follicles. Follicular count and atretic index were assessed besides, serum estradiol, progesterone, FSH and LH, C-reactive protein (CRP), malondialdehyde (MDA) and total antioxidant capacity (TAC) were measured. TCC offspring exhibited a significant delay in the onset of puberty and impedance of normal transition of the primordial follicles to more developed ones with altered cyctoarchitecture. Also, TCC decreased follicular count, proliferation and gonado-somatic index while it increased atretic index, apoptosis and AR of the granulosa cells along with disturbance of the feminine hormonal profile and oxidant/antioxidant balance. This study highlighted the potential long-term consequences of in utero and lactational exposure to TCC on the postnatal development of the ovary in rat offspring.
摘要：
三氯卡班(TCC),一种抗菌化合物，通常添加到各种家庭和个人卫生护理产品中，是最普遍的内分泌干扰物质（EDS）之一。进行这项研究是为了阐明子宫内和哺乳期暴露于TCC是否会对雌性大鼠后代的卵泡发育和青春期的开始产生不利影响。从妊娠第5天到出生后第21天（PND21），将20只怀孕的SpragueDawley大鼠平均分为对照组和TCCdam组（每天补充富含0.5mg/LTCC的饮用水）。雌性后代,20来自控制和20来自TCC大坝，分为4个亚组(PND21、PND28、PND35和PND42)。确定阴道开放的日期和第一发情周期。对子代的卵巢切片进行H&E染色和卵巢卵泡颗粒细胞上Ki67、Caspase-3和雄激素受体(AR)的免疫组织化学表达。此外，还评估了卵泡计数和闭锁指数，血清雌二醇，黄体酮,FSH和LH,C反应蛋白(CRP),测定丙二醛(MDA)和总抗氧化能力(TAC)。TCC后代在青春期的开始和原始卵泡正常过渡到更发达的卵泡的阻抗方面表现出明显的延迟，这些卵泡的细胞结构发生了改变。此外，TCC减少了卵泡计数，增殖和性腺-体细胞指数，而它增加了闭锁指数，颗粒细胞的凋亡和AR以及女性激素谱和氧化剂/抗氧化剂平衡的紊乱。这项研究强调了子宫内和哺乳期暴露于TCC对大鼠后代卵巢出生后发育的潜在长期后果。
