Abstract：
Women with premature ovarian insufficiency (POI) may be more vulnerable to a variety of health risks. To seek a new method to treat the disease, the effects of low-intensity pulsed ultrasound (LIPUS) on promoting repair of ovarian injury in female SD rats induced by 4-vinylcyclohexene diepoxide (VCD) were explored in this research. A total of 24 female SD rats were subjected to intraperitoneal injection of VCD to induce POI. Successful modeling was achieved in 22 rats, which were then randomized into VCD + LIPUS group (n = 13) and VCD group (n = 9). The control group (n = 5) was injected with equal normal saline. Hematoxylin and eosin staining, enzyme-linked immunosorbent assay, Western blot analysis, scanning electron microscope, immunohistochemistry, and terminal deoxynucleotidyl transferase-mediated nick end labeling assay were applied to detect the results. The results indicated that rats in the VCD group showed disorder in the estrous cycle, the number of atresia follicles and apoptosis granulosa cells increased (p < .05). After the LIPUS treatment, the estrous cycle recovered, the number of follicles increased (p < .05), the level of E2 and anti-Müllerian hormone enhanced (p < .05), and the follicle-stimulating hormone decreased (p < .05). The expression of NF-κB p65, TNFα, Bax, ATF4, and caspase-3 in ovarian tissue was significantly decreased (p < .05). These findings showed that LIPUS could promote the repair of the VCD-induced ovarian damage in SD rats, which has the potential to be further applied in the clinic.
摘要：
患有过早卵巢功能不全（POI）的女性可能更容易受到各种健康风险的影响。寻求治疗这种疾病的新方法，本研究探讨了低强度脉冲超声（LIPUS）对4-乙烯基环己烯二环氧化物（VCD）诱导的雌性SD大鼠卵巢损伤修复的影响。对24只雌性SD大鼠进行腹膜内注射VCD以诱导POI。22只大鼠成功建模,然后随机分为VCD+LIPUS组(n=13)和VCD组(n=9)。对照组(n=5)注射等量生理盐水。苏木精和伊红染色，酶联免疫吸附测定，蛋白质印迹分析,扫描电子显微镜，免疫组织化学，并应用末端脱氧核苷酸转移酶介导的缺口末端标记法检测结果。结果表明，VCD组大鼠出现发情周期紊乱，卵泡闭锁和颗粒细胞凋亡增加（p<0.05）。在LIPUS治疗之后,发情周期恢复了，卵泡数量增加（p<0.05），E2和抗苗勒管激素的水平提高（p<0.05），促卵泡激素下降（p<0.05）。NF-κBp65、TNFα的表达,Bax,卵巢组织中ATF4、caspase-3显著降低(p<0.05)。提示LIPUS能促进VCD诱导的SD大鼠卵巢损伤的修复,有可能进一步应用于临床。
