Abstract:
Accumulated lines of evidence have revealed that a large number of circular RNAs are produced in transcriptomes from fruit fly to mouse and human. Unlike linear RNAs shaped with 5\' cap and 3\' tail, circular RNAs are characterized by covalently closed loop structures without open terminals, thus required specific treatments for their identification and validation. Here, we describe a detailed pipeline for the characterization of circular RNAs. It has been successfully applied to the study of circular intronic RNAs (ciRNAs) derived from intron lariats and circular RNAs (circRNAs) produced from back spliced exons in human.
摘要:
积累的证据表明,从果蝇到小鼠和人的转录组中产生了大量环状RNA。与具有5个“帽”和3个“尾”形状的线性RNA不同,环状RNA的特征是没有开放末端的共价闭环结构,因此需要特定的治疗方法来进行鉴定和验证。这里,我们描述了环状RNA表征的详细流程。它已成功应用于人类内含子lariats衍生的环状内含子RNA(ciRNAs)和反向剪接外显子产生的环状RNA(circularRNAs)的研究。