Abstract：
Photovoltaic panels can be colonized by a highly diverse microbial diversity, despite life-threatening conditions. Although they are distributed worldwide, the microorganisms living on their surfaces have never been profiled in tropical regions using 16S rRNA high-throughput sequencing and PICRUst metagenome prediction of functional content. In this work, we investigated photovoltaic panels from two cities in southeast Brazil, Sorocaba and Itatiba, using these bioinformatics approach. Results showed that, despite significant differences in microbial diversity (p < 0.001), the taxonomic profile was very similar for both photovoltaic panels, dominated mainly by Proteobacteria, Bacteroidota and lower amounts of Cyanobacteria phyla. A predominance of Hymenobacter and Methylobacterium-Methylorubrum was observed at the genus level. We identified a microbial common core composed of Hymenobacter, Deinococcus, Sphingomonas, Methylobacterium-Methylorubrum, Craurococcus-Caldovatus, Massilia, Noviherbaspirillum and 1174-901-12 sharing genera. Predicted metabolisms focused on specific genes associated to radiation and desiccation resistance and pigments, were detected in members of the common core and among the most abundant genera. Our results suggested that taxonomic and functional profiles investigated were consistent with the harsh environment that photovoltaic panels represent. Moreover, the presence of stress genes in the predicted functional content was a preliminary evidence that microbes living there are a possibly source of metabolites with biotechnological interest.
摘要：
光伏板可以由高度多样化的微生物多样性定植，尽管有生命危险.尽管它们分布在世界各地，生活在其表面上的微生物从未在热带地区使用16SrRNA高通量测序和PICRUst宏基因组功能含量预测进行过分析。在这项工作中,我们调查了巴西东南部两个城市的光伏板，索罗卡巴和伊塔提巴,使用这些生物信息学方法。结果表明,尽管微生物多样性存在显著差异(p<0.001)，两种光伏板的分类特征非常相似，主要以变形杆菌为主，类细菌和低量的蓝细菌门。在属水平上观察到了淡膜菌和甲基细菌-甲基细菌的优势。我们确定了一个微生物的共同核心，异常球菌,鞘氨醇单胞菌,甲基细菌-甲基细菌，Craurococus-Caldovatus,Massilia,Noviherbaspirillum和1174-901-12共有属。预测的代谢集中在与辐射和干燥抗性和色素相关的特定基因上，在共同核心成员和最丰富的属中检测到。我们的结果表明，所研究的分类学和功能概况与光伏板所代表的恶劣环境一致。此外，在预测的功能内容中存在应激基因是一个初步证据，表明生活在那里的微生物可能是具有生物技术兴趣的代谢物来源。
