Abstract：
UNASSIGNED: Staphylococcus aureus is endowed with a repertoire of virulence factors potentially implicated in its pathogenicity and ability to cause invasive disease. The main objective of this study was to describe the bacterial genotype, including virulence genes and affiliation to clonal complexes (CCs), encountered in severe pneumonia.
UNASSIGNED: DNA microarray was used to analyse 18 S. aureus isolates from patients hospitalized with severe pneumonia between 2017 and 2019.
UNASSIGNED: Among 18 S. aureus isolates, 14 were methicillin-susceptible S. aureus (MSSA), and 4 methicillin-resistant S. aureus (MRSA). There were 14 community-acquired, 3 healthcare-associated, and 1 hospital-acquired infections. Different radiological presentations were observed: necrotizing pneumonia (n = 8, 44%), alveolar consolidation (n = 7, 39%), alveolar-interstitial infiltrates (n = 3, 17%). Sixteen patients (89%) required ICU hospitalization, 13 (72%) an invasive mechanical ventilation, and 12 (67%) a vasopressor support. Mortality affected 6 patients (33%). Panton-Valentine leukocidin (PVL), staphylococcal enterotoxins, toxic shock syndrome toxine-1 (TSST-1) encoding genes were documented in nine (50%), 12 (67%), one (6%) of the isolates, respectively. Accessory regulator gene group I was the most reported (n = 9, 50%) and was found in five deaths. The majority of isolates were affiliated to CC152 (n = 6), followed by CC15 (n = 3), CC45 (n = 2), CC30 (n = 2), CC1 (n = 2), CC8 (n = 1), CC9 (n = 1), and CC25 (n = 1). All the CC152 isolates were PVL-positive.
UNASSIGNED: CC152-PVL positive S. aureus strains were the most prevalent in severe pneumonia. Other virulence gene profiles were found coupled to additional clonal lineages. A genotyping strategy contributes to describe the current circulating strains and bacterial genetic backgrounds.
UNASSIGNED: DNA microarray was used to analyse 18 S. aureus isolates from patients hospitalized with severe pneumonia between 2017 and 2019.
UNASSIGNED: Among 18 S. aureus isolates, 14 were methicillin-susceptible S. aureus (MSSA), and 4 methicillin-resistant S. aureus (MRSA). There were 14 community-acquired, 3 healthcare-associated, and 1 hospital-acquired infections. Different radiological presentations were observed: necrotizing pneumonia (n = 8, 44%), alveolar consolidation (n = 7, 39%), alveolar-interstitial infiltrates (n = 3, 17%). Sixteen patients (89%) required ICU hospitalization, 13 (72%) an invasive mechanical ventilation, and 12 (67%) a vasopressor support. Mortality affected 6 patients (33%). Panton-Valentine leukocidin (PVL), staphylococcal enterotoxins, toxic shock syndrome toxine-1 (TSST-1) encoding genes were documented in nine (50%), 12 (67%), one (6%) of the isolates, respectively. Accessory regulator gene group I was the most reported (n = 9, 50%) and was found in five deaths. The majority of isolates were affiliated to CC152 (n = 6), followed by CC15 (n = 3), CC45 (n = 2), CC30 (n = 2), CC1 (n = 2), CC8 (n = 1), CC9 (n = 1), and CC25 (n = 1). All the CC152 isolates were PVL-positive.
UNASSIGNED: CC152-PVL positive S. aureus strains were the most prevalent in severe pneumonia. Other virulence gene profiles were found coupled to additional clonal lineages. A genotyping strategy contributes to describe the current circulating strains and bacterial genetic backgrounds.
摘要：
■金黄色葡萄球菌具有一系列可能与其致病性和引起侵袭性疾病的能力有关的毒力因子。这项研究的主要目的是描述细菌基因型，包括毒力基因和与克隆复合物（CC）的联系，在重症肺炎中遇到。
■DNA微阵列用于分析2017年至2019年重症肺炎住院患者的18株金黄色葡萄球菌分离株。
■在18株金黄色葡萄球菌中，14是甲氧西林敏感的金黄色葡萄球菌（MSSA），和4种耐甲氧西林金黄色葡萄球菌(MRSA)。有14个社区获得的，3医疗保健相关，1例医院感染。观察到不同的放射学表现：坏死性肺炎（n=8，44％），肺泡实变（n=7，39%），肺泡间质浸润（n=3，17％）。16例患者（89%）需要ICU住院治疗，13（72%）有创机械通气，和12（67%）血管加压药支持。死亡率影响6例(33%)。潘顿-瓦伦丁杀白细胞素(PVL)，葡萄球菌肠毒素,中毒性休克综合征毒素-1（TSST-1）编码基因在9个（50％）中被记录，12（67%），一个（6%）的分离株，分别。附件调节基因I组报道最多（n=9，50％），在5例死亡中发现。大多数分离株与CC152相关(n=6)，其次是CC15(n=3)，CC45（n=2），CC30（n=2），CC1（n=2），CC8（n=1），CC9（n=1），和CC25(n=1)。所有CC152分离株均为PVL阳性。
■CC152-PVL阳性金黄色葡萄球菌菌株在重症肺炎中最为普遍。发现其他毒力基因谱与其他克隆谱系偶联。基因分型策略有助于描述当前的循环菌株和细菌遗传背景。
■DNA微阵列用于分析2017年至2019年重症肺炎住院患者的18株金黄色葡萄球菌分离株。
■在18株金黄色葡萄球菌中，14是甲氧西林敏感的金黄色葡萄球菌（MSSA），和4种耐甲氧西林金黄色葡萄球菌(MRSA)。有14个社区获得的，3医疗保健相关，1例医院感染。观察到不同的放射学表现：坏死性肺炎（n=8，44％），肺泡实变（n=7，39%），肺泡间质浸润（n=3，17％）。16例患者（89%）需要ICU住院治疗，13（72%）有创机械通气，和12（67%）血管加压药支持。死亡率影响6例(33%)。潘顿-瓦伦丁杀白细胞素(PVL)，葡萄球菌肠毒素,中毒性休克综合征毒素-1（TSST-1）编码基因在9个（50％）中被记录，12（67%），一个（6%）的分离株，分别。附件调节基因I组报道最多（n=9，50％），在5例死亡中发现。大多数分离株与CC152相关(n=6)，其次是CC15(n=3)，CC45（n=2），CC30（n=2），CC1（n=2），CC8（n=1），CC9（n=1），和CC25(n=1)。所有CC152分离株均为PVL阳性。
■CC152-PVL阳性金黄色葡萄球菌菌株在重症肺炎中最为普遍。发现其他毒力基因谱与其他克隆谱系偶联。基因分型策略有助于描述当前的循环菌株和细菌遗传背景。
