UNASSIGNED: DNA microarray was used to analyse 18 S. aureus isolates from patients hospitalized with severe pneumonia between 2017 and 2019.
UNASSIGNED: Among 18 S. aureus isolates, 14 were methicillin-susceptible S. aureus (MSSA), and 4 methicillin-resistant S. aureus (MRSA). There were 14 community-acquired, 3 healthcare-associated, and 1 hospital-acquired infections. Different radiological presentations were observed: necrotizing pneumonia (n = 8, 44%), alveolar consolidation (n = 7, 39%), alveolar-interstitial infiltrates (n = 3, 17%). Sixteen patients (89%) required ICU hospitalization, 13 (72%) an invasive mechanical ventilation, and 12 (67%) a vasopressor support. Mortality affected 6 patients (33%). Panton-Valentine leukocidin (PVL), staphylococcal enterotoxins, toxic shock syndrome toxine-1 (TSST-1) encoding genes were documented in nine (50%), 12 (67%), one (6%) of the isolates, respectively. Accessory regulator gene group I was the most reported (n = 9, 50%) and was found in five deaths. The majority of isolates were affiliated to CC152 (n = 6), followed by CC15 (n = 3), CC45 (n = 2), CC30 (n = 2), CC1 (n = 2), CC8 (n = 1), CC9 (n = 1), and CC25 (n = 1). All the CC152 isolates were PVL-positive.
UNASSIGNED: CC152-PVL positive S. aureus strains were the most prevalent in severe pneumonia. Other virulence gene profiles were found coupled to additional clonal lineages. A genotyping strategy contributes to describe the current circulating strains and bacterial genetic backgrounds.
■DNA微阵列用于分析2017年至2019年重症肺炎住院患者的18株金黄色葡萄球菌分离株。
■在18株金黄色葡萄球菌中,14是甲氧西林敏感的金黄色葡萄球菌(MSSA),和4种耐甲氧西林金黄色葡萄球菌(MRSA)。有14个社区获得的,3医疗保健相关,1例医院感染。观察到不同的放射学表现:坏死性肺炎(n=8,44%),肺泡实变(n=7,39%),肺泡间质浸润(n=3,17%)。16例患者(89%)需要ICU住院治疗,13(72%)有创机械通气,和12(67%)血管加压药支持。死亡率影响6例(33%)。潘顿-瓦伦丁杀白细胞素(PVL),葡萄球菌肠毒素,中毒性休克综合征毒素-1(TSST-1)编码基因在9个(50%)中被记录,12(67%),一个(6%)的分离株,分别。附件调节基因I组报道最多(n=9,50%),在5例死亡中发现。大多数分离株与CC152相关(n=6),其次是CC15(n=3),CC45(n=2),CC30(n=2),CC1(n=2),CC8(n=1),CC9(n=1),和CC25(n=1)。所有CC152分离株均为PVL阳性。
■CC152-PVL阳性金黄色葡萄球菌菌株在重症肺炎中最为普遍。发现其他毒力基因谱与其他克隆谱系偶联。基因分型策略有助于描述当前的循环菌株和细菌遗传背景。