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Abstract：
BACKGROUND: Peripheral neuropathy (PN) is the damage and dysfunction of neurons of the peripheral nervous system. The present study was conducted to estimate the effectiveness of low-power laser therapy (LPLT) in the management of PN in a rats\' model.
METHODS: PN was induced by giving dichloroacetate (DCA) (250 mg/kg/day) for up to 12 weeks. Four groups of rats were used: control group, PN group, PN group treated with gabapentin and PN group treated with LPLT. The study was conducted for 8 weeks. The management of PN was estimated by behavioral tests which included hot plate and Morris water maze tests. Blood biochemical analysis were carried out.
RESULTS: Using of hot plate test indicated thermal hypoalgesia and using Morris water maze test showed cognitive decline in PN rats. Treatment with LPLT or gabapentin improved both the pain sensations and deteriorated memory that occurred in the PN rats. Biochemical analysis showed that LPLT significantly decreased the elevated beta-endorphin level in PN rats, while gabapentin could not reduce it. Treatment PN rats with LPLT or gabapentin shifted the high levels of TNF-α, IL-1β and IL-10 cytokines back to their normal values. Serum nitric oxide and MDA significantly increased in the PN group together with significant reduction in the rGSH level, these values were significantly improved by LPLT application while this was not the case with gabapentin treatment. Furthermore, treatment with gabapentin or LPLT significantly reduced serum ALAT and ASAT activities which are otherwise increased in the PN group. S100B, PGE2, total cholesterol, triglycerides, LDL-cholesterol, HDL-cholesterol, urea and creatinine showed insignificant changes among all groups.
CONCLUSIONS: Our results showed that treatment with LPLT is more efficient than gabapentin in ameliorating the peripheral neuropathy induced by xenobiotics.
METHODS: PN was induced by giving dichloroacetate (DCA) (250 mg/kg/day) for up to 12 weeks. Four groups of rats were used: control group, PN group, PN group treated with gabapentin and PN group treated with LPLT. The study was conducted for 8 weeks. The management of PN was estimated by behavioral tests which included hot plate and Morris water maze tests. Blood biochemical analysis were carried out.
RESULTS: Using of hot plate test indicated thermal hypoalgesia and using Morris water maze test showed cognitive decline in PN rats. Treatment with LPLT or gabapentin improved both the pain sensations and deteriorated memory that occurred in the PN rats. Biochemical analysis showed that LPLT significantly decreased the elevated beta-endorphin level in PN rats, while gabapentin could not reduce it. Treatment PN rats with LPLT or gabapentin shifted the high levels of TNF-α, IL-1β and IL-10 cytokines back to their normal values. Serum nitric oxide and MDA significantly increased in the PN group together with significant reduction in the rGSH level, these values were significantly improved by LPLT application while this was not the case with gabapentin treatment. Furthermore, treatment with gabapentin or LPLT significantly reduced serum ALAT and ASAT activities which are otherwise increased in the PN group. S100B, PGE2, total cholesterol, triglycerides, LDL-cholesterol, HDL-cholesterol, urea and creatinine showed insignificant changes among all groups.
CONCLUSIONS: Our results showed that treatment with LPLT is more efficient than gabapentin in ameliorating the peripheral neuropathy induced by xenobiotics.
摘要：
背景：周围神经病（PN）是周围神经系统神经元的损伤和功能障碍。本研究旨在评估低功率激光治疗（LPLT）在大鼠模型中治疗PN的有效性。
方法：通过给予二氯乙酸（DCA）（250mg/kg/天）长达12周诱导PN。使用四组大鼠:对照组,PN组,PN组采用加巴喷丁治疗，PN组采用LPLT治疗。该研究进行了8周。PN的管理是通过包括热板和Morris水迷宫测试在内的行为测试来估计的。进行血液生化分析。
结果：使用热板试验表明PN大鼠有热痛觉减退，使用Morris水迷宫试验表明认知功能下降。用LPLT或加巴喷丁治疗可改善PN大鼠的疼痛感觉和记忆力下降。生化分析表明,LPLT能显著降低PN大鼠β-内啡肽水平,而加巴喷丁不能减少它。用LPLT或加巴喷丁治疗PN大鼠改变了高水平的TNF-α，IL-1β和IL-10细胞因子恢复到正常值。PN组血清一氧化氮和MDA显著升高，rGSH水平显著降低，应用LPLT可显著改善这些值,而加巴喷丁治疗则并非如此.此外，加巴喷丁或LPLT治疗可显着降低血清ALAT和ASAT活性，而PN组则增加。S100B,PGE2,总胆固醇,甘油三酯，LDL-胆固醇,HDL-胆固醇,所有组的尿素和肌酐均无明显变化.
结论：我们的结果表明，LPLT治疗比加巴喷丁更有效地改善外源性物质引起的周围神经病变。
方法：通过给予二氯乙酸（DCA）（250mg/kg/天）长达12周诱导PN。使用四组大鼠:对照组,PN组,PN组采用加巴喷丁治疗，PN组采用LPLT治疗。该研究进行了8周。PN的管理是通过包括热板和Morris水迷宫测试在内的行为测试来估计的。进行血液生化分析。
结果：使用热板试验表明PN大鼠有热痛觉减退，使用Morris水迷宫试验表明认知功能下降。用LPLT或加巴喷丁治疗可改善PN大鼠的疼痛感觉和记忆力下降。生化分析表明,LPLT能显著降低PN大鼠β-内啡肽水平,而加巴喷丁不能减少它。用LPLT或加巴喷丁治疗PN大鼠改变了高水平的TNF-α，IL-1β和IL-10细胞因子恢复到正常值。PN组血清一氧化氮和MDA显著升高，rGSH水平显著降低，应用LPLT可显著改善这些值,而加巴喷丁治疗则并非如此.此外，加巴喷丁或LPLT治疗可显着降低血清ALAT和ASAT活性，而PN组则增加。S100B,PGE2,总胆固醇,甘油三酯，LDL-胆固醇,HDL-胆固醇,所有组的尿素和肌酐均无明显变化.
结论：我们的结果表明，LPLT治疗比加巴喷丁更有效地改善外源性物质引起的周围神经病变。
