PDF(Pubmed)
Abstract：
The presence of circulating microbiome in blood has been reported in both physiological and pathological conditions, although its origins, identities and function remain to be elucidated. This study aimed to investigate the presence of blood microbiome by quantitative real-time PCRs targeting the 16S rRNA gene. To our knowledge, this is the first study in which the circulating microbiome has been analyzed in such a large sample of individuals since the study was carried out on 1285 Randomly recruited Age-Stratified Individuals from the General population (RASIG). The samples came from several different European countries recruited within the EU Project MARK-AGE in which a series of clinical biochemical parameters were determined. The results obtained reveal an association between microbial DNA copy number and geographic origin. By contrast, no gender and age-related difference emerged, thus demonstrating the role of the environment in influencing the above levels independent of age and gender at least until the age of 75. In addition, a significant positive association was found with Free Fatty Acids (FFA) levels, leukocyte count, insulin, and glucose levels. Since these factors play an essential role in both health and disease conditions, their association with the extent of the blood microbiome leads us to consider the blood microbiome as a potential biomarker of human health.
摘要：
血液中循环微生物组的存在已经报道在生理和病理条件下，虽然它的起源，身份和功能仍有待阐明。本研究旨在通过靶向16SrRNA基因的定量实时PCR来研究血液微生物组的存在。据我们所知,这是该研究是对来自普通人群的1285名随机招募的年龄分层个体(RASIG)进行研究以来,首次在如此大的个体样本中分析循环微生物组.样品来自欧盟MARK-AGE项目中招募的几个不同的欧洲国家，其中确定了一系列临床生化参数。获得的结果揭示了微生物DNA拷贝数与地理起源之间的关联。相比之下,没有出现性别和年龄相关的差异，因此，至少在75岁之前，证明了环境在影响上述水平方面的作用，而与年龄和性别无关。此外,发现与游离脂肪酸（FFA）水平呈显著正相关，白细胞计数,胰岛素，和葡萄糖水平。由于这些因素在健康和疾病状况中起着至关重要的作用，它们与血液微生物组程度的关联使我们将血液微生物组视为人类健康的潜在生物标志物.
