PDF(Pubmed)
Abstract：
The solute carrier family has been reported to play critical roles in the progression of several cancers; however, the relationship between solute carrier family 12 member 8 (SLC12A8) and bladder cancer (BC) has not been clearly confirmed. This study explores the prognostic value of SLC12A8 for BC and its correlation with immune cell infiltration. We found that the expression of SLC12A8 mRNA was significantly overexpressed in BC tissues compared with noncancerous tissues in multiple public databases, and the result was validated using real-time PCR and immunohistochemistry (IHC). The Kaplan-Meier method and Cox proportional hazards models were used to evaluate the prognostic value of SLC12A8 for BC. The high expression of SLC12A8 led to a shorter overall survival time and was an unfavorable prognostic biomarker for BC. The mechanisms of SLC12A8 promoting tumorigenesis were investigated by Gene Set Enrichment Analysis (GSEA). Moreover, the correlations of SLC12A8 expression with the tumor-infiltrating immune cells (TICs) in BC were explored using TIMER 2.0 and CIBERSORT. SLC12A8 was associated with CD4+ T cells, dendritic cells, neutrophils, and macrophages infiltration. The expression of SLC12A8 was positively correlated with crucial immune checkpoint molecules. In conclusion, SLC12A8 might be an unfavorable prognostic biomarker in BC related to tumor immune cell infiltration.
摘要：
据报道，溶质载体家族在几种癌症的进展中起着关键作用；然而，溶质载体家族12成员8（SLC12A8）与膀胱癌（BC）之间的关系尚未明确证实。本研究探讨SLC12A8对BC的预后价值及其与免疫细胞浸润的相关性。我们发现，在多个公共数据库中，与非癌组织相比，在BC组织中SLC12A8mRNA的表达显著过表达，并使用实时PCR和免疫组织化学（IHC）验证结果。采用Kaplan-Meier法和Cox比例风险模型评价SLC12A8对BC的预后价值。SLC12A8的高表达导致较短的总体存活时间,并且是BC的不利的预后生物标志物。通过基因集富集分析（GSEA）研究了SLC12A8促进肿瘤发生的机制。此外，使用TIMER2.0和CIBERSORT研究了SLC12A8表达与BC中肿瘤浸润性免疫细胞（TIC）的相关性。SLC12A8与CD4+T细胞相关,树突状细胞,中性粒细胞，和巨噬细胞浸润。SLC12A8的表达与关键免疫检查点分子呈正相关。总之,SLC12A8可能是与肿瘤免疫细胞浸润相关的BC中不良预后生物标志物。
