PDF(Pubmed)
Abstract：
Matrix metalloproteinases (MMPs) are crucial for tissue remodeling and repair and are expressed in diverse infections, whereas tissue inhibitors of metalloproteinases (TIMPs) are endogenous inhibitors of MMPs. However, the interaction of MMPs and TIMPs in tuberculous lymphadenitis (TBL), an extra-pulmonary form of tuberculosis (EPTB) and helminth (Hel+) coinfection is not known. Therefore, this present study investigates the levels of circulating MMPs (1, 2, 3, 7, 8, 9, 12, 13) and TIMPs (1, 2, 3, 4) in TBL individuals with helminth (Strongyloides stercoralis [Ss], hereafter Hel+) coinfection and without helminth coinfection (hereafter, Hel-). In addition, we have also carried out the regression analysis and calculated the MMP/TIMP ratios between the two study groups. We describe that the circulating levels of MMPs (except MMP-8 and MMP-12) were elevated in TBL-Hel+ coinfected individuals compared to TBL-Hel- individuals. Similarly, the systemic levels of TIMPs (1, 2, 3, 4) were increased in TBL-Hel+ compared to TBL-Hel- groups indicating that it is a feature of helminth coinfection per se. Finally, our multivariate analysis data also revealed that the changes in MMPs and TIMPs were independent of age, sex, and culture status between TBL-Hel+ and TBL-Hel- individuals. We show that the MMP-2 ratio with all TIMPs were significantly associated with TBL-helminth coinfection. Thus, our results describe how helminth infection has a profound effect on the pathogenesis of TBL and that both MMPs and TIMPs could dampen the immunity against the TBL-Hel+ coinfected individuals.
摘要：
基质金属蛋白酶（MMPs）对于组织重塑和修复至关重要，并在多种感染中表达。而金属蛋白酶组织抑制剂（TIMPs）是MMPs的内源性抑制剂。然而,结核性淋巴结炎（TBL）中MMPs和TIMPs的相互作用，肺外形式的肺结核（EPTB）和蠕虫（Hel）共感染尚不清楚。因此,本研究调查了患有蠕虫的TBL个体中循环MMPs（1、2、3、7、8、9、12、13）和TIMPs（1、2、3、4）的水平以下为Hel+)共感染和无蠕虫共感染(以下为Hele-).此外,我们还进行了回归分析,并计算了两个研究组之间的MMP/TIMP比值.我们描述了与TBL-Hel-个体相比，TBL-Hel共感染的个体中MMP（MMP-8和MMP-12除外）的循环水平升高。同样，与TBL-Hel组相比，TBL-Hel组的TIMPs的全身水平（1、2、3、4）升高，表明它本身是蠕虫共感染的特征。最后,我们的多变量分析数据还显示，MMP和TIMPs的变化与年龄无关，性别,以及TBL-Hel+和TBL-Hel-个体之间的培养状况。我们表明，所有TIMPs的MMP-2比率与TBL蠕虫共感染显着相关。因此，我们的结果描述了蠕虫感染如何对TBL的发病机制产生深远的影响，并且MMPs和TIMPs都可以降低对TBL-Hel共感染个体的免疫力。
