PDF(Pubmed)
Abstract：
Anti-G antibody mimics the reactivity pattern of coexistent anti-D and anti-C. Differentiating between the two is significant in antenatal females where the decision to administer RhD prophylaxis is based on the presence or absence of anti-D antibody. The aim of reporting this serological challenge is to emphasize the need for phenotyping red cells for sourcing appropriate in house red cell reagents and to help transfusion services sharpen problem-solving skills. A 26-year-old pregnant female with a complicated obstetric history and a positive indirect antiglobulin test presented to the hospital for antenatal assessment at 24 weeks. A positive antibody screen warranted identification of the implicating antibodies. Since identification was suggestive of multiple alloantibodies whose specificities could not be confirmed, step-wise sequential adsorption and elution was required. Anti-D, anti-C, and anti-E antibodies were identified in patient plasma with titers of 1024, 4, and 32, respectively. The absence of anti-G was also confirmed. Multiple alloantibodies can pose a challenge to transfusion services. However, with the help of select cells, phenotyping, adsorption elution studies, and phenotyped donor units; solving complex serological cases can be accomplished.
摘要：
抗G抗体模拟共存的抗D和抗C的反应模式。在产前女性中，两者之间的区别很重要，在产前女性中，进行RhD预防的决定是基于抗D抗体的存在与否。报告这一血清学挑战的目的是强调需要对红细胞进行表型分型，以便在室内采购适当的红细胞试剂，并帮助输血服务提高解决问题的能力。一名26岁的孕妇，其产科病史复杂，间接抗球蛋白试验阳性，在24周时被送往医院进行产前评估。阳性抗体筛选保证了潜在抗体的鉴定。由于鉴定提示多种同种抗体的特异性无法确认，需要分步顺序吸附和洗脱。反D,反C,在患者血浆中鉴定出抗E抗体，滴度分别为1024、4和32。也证实了抗G的不存在。多种同种抗体可能对输血服务构成挑战。然而,在选择单元格的帮助下，表型，吸附洗脱研究，和表型供体单位；可以解决复杂的血清学病例。
