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Abstract：
OBJECTIVE: Peritoneal endometriosis is a chronic inflammatory disease particularly associated with macrophages. Of note, adipose tissues with fibrotic changes in the context of peritoneal endometriotic lesions are often observed during surgery. However, the characteristics of fibrotic adipose tissues in endometriosis are still unknown. In this study, we investigated the inflammatory status of retroperitoneal adipose tissues adjacent to pelvic endometriotic lesions.
METHODS: Thirty-two patients who underwent surgical treatment were assigned to either the endometriosis (n = 16) or the control (n = 16) groups. Retroperitoneal adipose tissues around the uterus were collected from patients in both groups. Fibrosis was evaluated via Masson\'s trichrome staining. Macrophage infiltration, the expression of fatty acid-binding protein 4 (FABP4), and angiogenesis in the retroperitoneal adipose tissues were evaluated via immunohistochemistry. The mRNA expression levels of cytokines was also evaluated in the adipose tissues using real-time PCR.
RESULTS: There was more fibrosis and angiogenesis in the adipose tissues adjacent to the endometriotic lesions with a significantly higher level of infiltration of macrophages and a predominance of the M1 type in the endometriosis group compared to the control group. In addition, FABP4 positivity in the adipose tissues of the peritoneum was significantly higher in the endometriosis group versus the control group. Moreover, the mRNA expression levels of FABP4, VEGF, and proinflammatory cytokines were also significantly higher in the endometriosis group.
CONCLUSIONS: Altogether, our results showed that the adipose tissue adjacent to endometriotic lesions are inflamed with fibrosis and angiogenesis.
METHODS: Thirty-two patients who underwent surgical treatment were assigned to either the endometriosis (n = 16) or the control (n = 16) groups. Retroperitoneal adipose tissues around the uterus were collected from patients in both groups. Fibrosis was evaluated via Masson\'s trichrome staining. Macrophage infiltration, the expression of fatty acid-binding protein 4 (FABP4), and angiogenesis in the retroperitoneal adipose tissues were evaluated via immunohistochemistry. The mRNA expression levels of cytokines was also evaluated in the adipose tissues using real-time PCR.
RESULTS: There was more fibrosis and angiogenesis in the adipose tissues adjacent to the endometriotic lesions with a significantly higher level of infiltration of macrophages and a predominance of the M1 type in the endometriosis group compared to the control group. In addition, FABP4 positivity in the adipose tissues of the peritoneum was significantly higher in the endometriosis group versus the control group. Moreover, the mRNA expression levels of FABP4, VEGF, and proinflammatory cytokines were also significantly higher in the endometriosis group.
CONCLUSIONS: Altogether, our results showed that the adipose tissue adjacent to endometriotic lesions are inflamed with fibrosis and angiogenesis.
摘要：
目的：腹膜子宫内膜异位症是一种特别与巨噬细胞相关的慢性炎症性疾病。值得注意的是，在腹膜子宫内膜异位病变的背景下，经常在手术期间观察到具有纤维化变化的脂肪组织。然而,子宫内膜异位症的纤维化脂肪组织特征尚不清楚。在这项研究中,我们调查了盆腔子宫内膜异位病灶附近腹膜后脂肪组织的炎症状态.
方法：32例接受手术治疗的患者被分为子宫内膜异位症组（n=16）或对照组（n=16）。收集两组患者子宫周围的腹膜后脂肪组织。纤维化通过Masson三色染色评估。巨噬细胞浸润,脂肪酸结合蛋白4（FABP4）的表达，通过免疫组织化学评估腹膜后脂肪组织中的血管生成。还使用实时PCR评估脂肪组织中细胞因子的mRNA表达水平。
结果：与对照组相比，子宫内膜异位症组子宫内膜异位症病灶附近脂肪组织中纤维化和血管生成更多，巨噬细胞浸润水平明显更高，M1型占主导地位。此外,子宫内膜异位症组腹膜脂肪组织中FABP4阳性明显高于对照组。此外，FABP4、VEGF、和促炎细胞因子也显著高于子宫内膜异位症组。
结论：总而言之，我们的研究结果表明，子宫内膜异位病灶附近的脂肪组织发炎并伴有纤维化和血管生成。
方法：32例接受手术治疗的患者被分为子宫内膜异位症组（n=16）或对照组（n=16）。收集两组患者子宫周围的腹膜后脂肪组织。纤维化通过Masson三色染色评估。巨噬细胞浸润,脂肪酸结合蛋白4（FABP4）的表达，通过免疫组织化学评估腹膜后脂肪组织中的血管生成。还使用实时PCR评估脂肪组织中细胞因子的mRNA表达水平。
结果：与对照组相比，子宫内膜异位症组子宫内膜异位症病灶附近脂肪组织中纤维化和血管生成更多，巨噬细胞浸润水平明显更高，M1型占主导地位。此外,子宫内膜异位症组腹膜脂肪组织中FABP4阳性明显高于对照组。此外，FABP4、VEGF、和促炎细胞因子也显著高于子宫内膜异位症组。
结论：总而言之，我们的研究结果表明，子宫内膜异位病灶附近的脂肪组织发炎并伴有纤维化和血管生成。
