Abstract：
Due to the emergence of multidrug-resistant bacteria, bacteriophages have become a viable alternative in controlling bacterial growth or biofilm formation. Biofilm is formed by extracellular polymeric substances (EPS) and is one of the factors responsible for increasing bacterial resistance. Bacteriophages have been studied as a bacterial control agent by use of phage enzymes or due to their bactericidal activities. A specific phage against Serratia marcescens was isolated in this work and was evaluated its biological and genomic aspects. The object of this study was UFV01, a bacteriophage belonging to the Podoviridae family, genus Teseptimavirus (group of lytic viruses), specific to the species S. marcescens, which may be related to several amino acid substitutions in the virus tail fibers. Despite this high specificity, the phage reduced the biofilm formation of several Escherichia coli strains without infecting them. UFV01 presents a relationship with phages of the genus Teseptimavirus, although it does not infect any of the E. coli strains evaluated, as these others do. All the characteristics make the phage an interesting alternative in biofilm control in hospital environments since small breaks in the biofilm matrix can lead to a complete collapse.
摘要：
由于多重耐药细菌的出现，噬菌体已成为控制细菌生长或生物膜形成的可行替代品。生物膜由胞外聚合物（EPS）形成，是增加细菌耐药性的因素之一。已经通过使用噬菌体酶或由于其杀菌活性将噬菌体作为细菌控制剂进行了研究。在这项工作中分离了针对粘质沙雷氏菌的特异性噬菌体，并对其生物学和基因组方面进行了评估。这项研究的对象是UFV01，一种属于Podoviridae家族的噬菌体，Teseptimavirus(groupoflyticvirus),特定于粘质链球菌，这可能与病毒尾纤维中的几个氨基酸取代有关。尽管这种高特异性,噬菌体减少了几种大肠杆菌菌株的生物膜形成，而不感染它们。UFV01表示与Teseptimavirus属噬菌体的关系，尽管它不感染任何评估的大肠杆菌菌株，就像其他人一样。所有特征使噬菌体成为医院环境中生物膜控制中的有趣替代品，因为生物膜基质中的小破裂会导致完全崩溃。
