Abstract:
Antimicrobial photodynamic therapy (aPDT) is an alternative approach. The current study aimed to investigate the efficacy of aPDT with indocyanine green (ICG) against two Candida albicans (C. albicans) strains. In this in vitro study, the inoculum of standard ATCC 10,231 (S) and fluconazole-resistant (FR) strains were adjusted to the turbidity of a 0.5 McFarland standard. Each strain was allocated into 4 groups: S1 and FR1) control groups, S2 and FR2) ICG-treated groups (1 µg/mL), S3 and FR3) laser-irradiated groups (wavelength: 810 nm; mode: continuous-wave; output power: 300 mW; spot size: 4.5 mm; exposure time: 120 s; radiation dose: 228 J/cm2), S4 and FR4) ICG-mediated-aPDT groups. After treatments, the number of colony-forming units per milliliter (CFU/mL) was calculated. Using the XTT reduction assay, the effects of each treatment on Candida biofilm formation were evaluated. Data were analyzed using SPSS software version 22. In both strains, the maximum number of CFUs was observed in the control group, followed by ICG-treated, laser-irradiated, and ICG-mediated-aPDT groups. In ATCC 10,231 strain, the XTT assay exhibited significant difference between ICG-mediated-aPDT and control groups (p < 0.0001). However, the ICG, laser, and ICG-mediated-aPDT groups in fluconazole-resistant strain showed significant differences when compared with the control (p < 0.05). The mean Candida CFUs and the XTT assay did not show any significant difference between the ATCC 10,231 and fluconazole-resistant strains with respect to each treatment. Data suggest ICG-mediated-aPDT could diminish Candida CFUs in laboratory; however, further studies are warranted to confirm its efficacy and safety to be applied in clinics.
摘要:
抗菌光动力疗法(aPDT)是一种替代方法。目前的研究旨在研究含吲哚菁绿(ICG)的aPDT对两种白色念珠菌(C.白色念珠菌)菌株。在这项体外研究中,将标准ATCC10,231(S)和氟康唑耐药(FR)菌株的接种物调节至0.5McFarland标准的浊度。每个菌株分为4组:S1和FR1)对照组,S2和FR2)ICG治疗组(1µg/mL),S3和FR3)激光照射组(波长:810nm;模式:连续波;输出功率:300mW;光斑尺寸:4.5mm;曝光时间:120s;辐射剂量:228J/cm2),S4和FR4)ICG介导的aPDT基团。治疗后,计算每毫升菌落形成单位数(CFU/mL).使用XTT还原测定,评估了每种处理对念珠菌生物膜形成的影响.使用SPSS软件版本22对数据进行分析。在这两种菌株中,对照组观察到CFU的最大数量,其次是ICG治疗,激光照射,和ICG介导的aPDT组。在ATCC10,231菌株中,XTT测定在ICG介导的aPDT和对照组之间显示出显著差异(p<0.0001)。然而,ICG,激光,与对照组相比,氟康唑耐药菌株和ICG介导的aPDT组显示出显着差异(p<0.05)。平均念珠菌CFU和XTT测定在ATCC10,231和氟康唑抗性菌株之间关于每种处理没有显示任何显著差异。数据表明ICG介导的aPDT可以减少实验室中的念珠菌CFU;然而,有必要进一步研究以确认其在临床中应用的有效性和安全性。