Abstract：
A chemiluminometric method is introduced for the determination of the stress biomarker, 3-nitrotyrosine (3-NT) based on the H2O2-NaIO4 reaction enhanced by cobalt and nitrogen-doped carbon dots (Co,N-CDs). In this chemiluminescence (CL) system, the emission proved to be originated from the excited-state Co,N-CDs (λmax = 504 nm). Comparing the effect of Co,N-CDs with that of some other metal ion-doped CDs and undoped CDs indicated the high efficiency of Co,N-CDs in the CL amplification (about 1980-fold). This was attributed to the fact that Co,N-CDs, in addition to other functions, could act as catalytic center, to accelerate the decomposition of H2O2 and to increase the number of hydroxyl radicals. It was found that 3-NT inhibits the action of Co,N-CDs by an electron transfer process, leading to a decline in the CL intensity of the system. Therefore, a new CL sensing platform was introduced for the assay of 3-NT in the range 5.0 to 300 nM with a detection limit of 1.5 nM. The probe was utilized for the analysis of biological samples.
摘要：
介绍了一种用于确定应激生物标志物的化学发光方法。基于钴和氮掺杂碳点增强的H2O2-NaIO4反应的3-硝基酪氨酸（3-NT）（Co，N-CD)。在这种化学发光（CL）系统中，发射被证明是来自激发态的Co，N-CD(λmax=504nm)。比较Co的效果，N-CD与其他一些金属离子掺杂的CD和未掺杂的CD表示Co的高效率，CL扩增中的N-CD(约1980倍)。这归因于这样一个事实，即Co，N-CD,除了其他功能，可以充当催化中心，以加速H2O2的分解和增加羟基自由基的数量。发现3-NT抑制Co的作用，N-CD通过电子转移过程，导致系统CL强度下降。因此,引入了一种新的CL传感平台,用于测定范围为5.0~300nM的3-NT,检出限为1.5nM.该探针用于生物样品的分析。
