PDF(Pubmed)
Abstract：
Banana is one of the most important fruit crops consumed globally owing to its high nutritional value. Previously, we demonstrated that the ripe pulp of the banana cultivar (cv.) Nendran (AAB) contained a high amount of pro-vitamin A carotenoids. However, the molecular factors involved in the ripening process in Nendran fruit are unexplored. Hence, we commenced a transcriptome study by using the Illumina HiSeq 2500 at two stages i.e. unripe and ripe fruit-pulp of Nendran. Overall, 3474 up and 4727 down-regulated genes were obtained. A large number of identified transcripts were related to genes involved in ripening, cell wall degradation and aroma formation. Gene ontology analysis highlighted differentially expressed genes that play a key role in various pathways. These pathways were mainly linked to cellular, molecular and biological processes. The present transcriptome study also reveals a crucial role of up-regulated carotenoid biosynthesis pathway genes namely, lycopene beta cyclase and geranylgeranyl pyrophosphate synthase at the ripening stage. Genes related to the ripening and other processes like aroma and flavor were highly expressed in the ripe pulp. Expression of numerous transcription factor family genes was also identified. This study lays a path towards understanding the ripening, carotenoid accumulation and other related processes in banana.
摘要：
香蕉因其营养价值高，是全球消费最重要的水果作物之一。以前,我们证明了香蕉品种的成熟果肉（cv。）Nendran（AAB）含有大量的前维生素A类胡萝卜素。然而,Nendran果实成熟过程中涉及的分子因素尚未研究。因此，我们通过在两个阶段使用IlluminaHiSeq2500开始了转录组研究，即Nendran的未成熟和成熟果肉。总的来说,获得了3474个上调基因和4727个下调基因。大量鉴定的转录本与参与成熟的基因有关，细胞壁降解和香气形成。基因本体论分析强调了在各种途径中起关键作用的差异表达基因。这些通路主要与细胞有关,分子和生物过程。本转录组研究还揭示了上调类胡萝卜素生物合成途径基因的关键作用，即成熟阶段的番茄红素β环化酶和香叶基香叶基焦磷酸合酶。与成熟和香气和风味等其他过程相关的基因在成熟果肉中高度表达。还鉴定了许多转录因子家族基因的表达。这项研究为理解成熟奠定了基础，类胡萝卜素在香蕉中的积累和其他相关过程。
