PDF(Pubmed)
Abstract：
Diabetic cardiomyopathy is a common complication of diabetes, in which endoplasmic reticulum stress (ERS) serves an important role. Rutin can treat the myocardial dysfunction of diabetic rats. However, to the best of our knowledge, studies on the effects of Rutin on myocardial injury caused by diabetes from the perspective of ERS have not previously been reported. In the present study, the role of rutin in the regulation of ERS in myocardial injury was assessed. Different high glucose concentrations were used to treat H9C2 myoblast cells to establish a myocardial damage model. A cell counting kit-8 assay was used to determine cell viability. A lactate dehydrogenase kit was used to detect cytotoxicity. Apoptosis levels were determined using a TUNEL assay. Western blotting was used to determine the expression levels of apoptosis-related proteins and ERS-related proteins, including heat shock protein A family member 5, inositol-requiring enzyme-1α, X-box binding protein 1, activating transcription factor 6, C/EBP-homologous protein (CHOP), cleaved caspase-12 and caspase-12. The anti-apoptotic and anti-ERS effects of Rutin on H9C2 cardiac cells induced by high glucose were examined after the administration of the ERS activator thapsigargin (TG). The results indicated that rutin could dose-dependently inhibit the level of apoptosis and ERS induced by high glucose in H9C2 cells. After administration of the ERS activator TG, it was demonstrated that TG could reverse the anti-apoptotic and anti-ERS effects of rutin on H9C2 cells stimulated with high glucose. Collectively, the present results suggested that rutin may alleviate cardiomyocyte model cell injury induced by high glucose through the inhibition of apoptosis and ERS.
摘要：
糖尿病心肌病是糖尿病的常见并发症,其中内质网应激（ERS）起重要作用。芦丁可以治疗糖尿病大鼠心肌功能障碍。然而,据我们所知,从ERS的角度研究芦丁对糖尿病引起的心肌损伤的影响尚未见报道。在本研究中,评估了芦丁在调节心肌损伤ERS中的作用.用分歧高糖浓度处置H9C2成肌细胞树立心肌毁伤模子。使用细胞计数试剂盒-8测定来确定细胞活力。乳酸脱氢酶试剂盒用于检测细胞毒性。使用TUNEL测定法测定细胞凋亡水平。蛋白质印迹法检测细胞凋亡相关蛋白和ERS相关蛋白的表达水平,包括热休克蛋白A家族成员5，需要肌醇的酶-1α，X-box结合蛋白1,转录激活因子6,C/EBP同源蛋白(CHOP),切割的caspase-12和caspase-12。在施用ERS激活剂thapsigargin（TG）后，检查了芦丁对高葡萄糖诱导的H9C2心肌细胞的抗凋亡和抗ERS作用。结果表明，芦丁可以剂量依赖性地抑制高糖诱导的H9C2细胞凋亡和ERS的水平。服用ERS激活剂TG后，结果表明，TG可以逆转芦丁对高糖刺激的H9C2细胞的抗凋亡和抗ERS作用。总的来说，提示芦丁可能通过抑制细胞凋亡和ERS减轻高糖诱导的心肌细胞损伤。
