PDF(Sci-hub)
Abstract：
Air pollution exposure during pregnancy may be a risk factor for altered immune maturation in the offspring. We investigated the association between ambient air pollutants during pregnancy and cell populations in cord blood from babies born to mothers with asthma enrolled in the Breathing for Life Trial. For each patient (n = 91), daily mean ambient air pollutant levels were extracted during their entire pregnancy for sulfur dioxide (SO2), nitric oxide, nitrogen dioxide, carbon monoxide, ozone, particulate matter <10 μm (PM10) or <2.5 μm (PM2.5), humidity, and temperature. Ninety-one cord blood samples were collected, stained, and assessed using fluorescence-activated cell sorting (FACS). Principal Component (PC) analyses of both air pollutants and cell types with linear regression were employed to define associations. Considering risk factors and correlations between PCs, only one PC from air pollutants and two from cell types were statistically significant. PCs from air pollutants were characterized by higher PM2.5 and lower SO2 levels. PCs from cell types were characterized by high numbers of CD8 T cells, low numbers of CD4 T cells, and by high numbers of plasmacytoid dendritic cells (pDC) and low numbers of myeloid DCs (mDCs). PM2.5 levels during pregnancy were significantly associated with high numbers of pDCs (p = 0.006), and SO2 with high numbers of CD8 T cells (p = 0.002) and low numbers of CD4 T cells (p = 0.011) and mDCs (p = 4.43 × 10-6) in cord blood. These data suggest that ambient SO2 and PM2.5 exposure are associated with shifts in cord blood cell types that are known to play significant roles in inflammatory respiratory disease in childhood.
摘要：
怀孕期间的空气污染暴露可能是后代免疫成熟改变的危险因素。我们调查了怀孕期间的环境空气污染物与参加生命呼吸试验的哮喘母亲所生的婴儿脐带血细胞群之间的关系。对于每位患者（n=91），在整个怀孕期间提取了每日平均环境空气污染物水平的二氧化硫（SO2），一氧化氮,二氧化氮,一氧化碳,臭氧,颗粒物<10μm(PM10)或<2.5μm(PM2.5)，湿度,湿度和温度。收集了91份脐带血样本，染色,并使用荧光激活细胞分选(FACS)进行评估。采用线性回归的空气污染物和细胞类型的主成分（PC）分析来定义关联。考虑到PC之间的风险因素和相关性，只有一种来自空气污染物的PC和两种来自细胞类型的PC具有统计学意义。来自空气污染物的PC的特征在于较高的PM2.5和较低的SO2水平。来自细胞类型的PC的特征是大量的CD8T细胞，CD4T细胞数量少，以及大量的浆细胞样树突状细胞（pDC）和少量的髓样DC（mDC）。怀孕期间的PM2.5水平与高pDCs数量显着相关（p=0.006），和SO2，脐带血中CD8T细胞数量高（p=0.002），CD4T细胞数量低（p=0.011）和mDCs数量低（p=4.43×10-6）。这些数据表明，环境中的SO2和PM2.5暴露与脐带血细胞类型的变化有关，而脐带血细胞类型在儿童的炎症性呼吸系统疾病中起着重要作用。
