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Abstract：
The aim of the study was to evaluate if a pre-incisional N-acetylcysteine (NAC) treatment altered the process of wound healing in a rat model. The dorsal skin of 24 Sprague-Dawley rats was incised in six locations. Before the incisions were made, skin was injected either with lidocaine and epinephrine (one side) or with these agents supplemented with 0.015%, 0.03%, or 0.045% NAC (contralaterally). Photographic documentation of the wound healing process was made at 11 time points. Rats were sacrificed 3, 7, 14, or 60 days after incision to excise scars for histological analysis. They included: Abramov scale scoring, histomorphometry analysis, and collagen fiber arrangement assessment. Skin pretreated with 0.03% NAC produced the shortest scars at all analyzed time points, though this result was statistically insignificant. At this NAC concentration the scars had smaller areas on the third day and were narrower on the day 4 compared with all the other groups (p < 0.05). On day 7, at the same concentration of NAC, the scars had a higher superficial concentration index (p = 0.03) and larger dermal proliferation area (p = 0.04). NAC addition to pre-incisional anesthetic solution decreased wound size and width at an early stage of scar formation at all concentrations; however, with optimal results at 0.03% concentration.
摘要：
研究的目的是评估切口前N-乙酰半胱氨酸（NAC）治疗是否改变了大鼠模型中伤口愈合的过程。在六个位置切开了24只Sprague-Dawley大鼠的背部皮肤。在切开切口之前，皮肤注射利多卡因和肾上腺素（一侧）或补充了0.015%的这些药物，0.03%,或0.045%NAC（对侧）。在11个时间点进行伤口愈合过程的照相记录。在切口后3、7、14或60天处死大鼠以切除疤痕用于组织学分析。其中包括：Abramov量表评分，组织形态计量学分析,和胶原纤维排列评估。用0.03%NAC预处理的皮肤在所有分析的时间点产生最短的疤痕，尽管这一结果在统计上微不足道。在该NAC浓度下,与所有其他组相比,疤痕在第三天具有更小的面积,并且在第4天更窄(p<0.05)。在第7天，在相同浓度的NAC下，疤痕的表面浓度指数较高（p=0.03），真皮增殖面积较大（p=0.04）。在所有浓度下，切口前麻醉溶液中添加NAC可在瘢痕形成的早期减少伤口的大小和宽度；然而，最佳结果在0.03%浓度。
