PDF(Pubmed)
Abstract：
UNASSIGNED: Acute lymphoblastic leukemia (ALL) is a malignant disease most commonly diagnosed in adolescents and young adults. This study aimed to explore potential signatures and their functions for ALL.
UNASSIGNED: Differentially expressed mRNAs (DEmRNAs) and differentially expressed long non-coding RNAs (DElncRNAs) were identified for ALL from The Cancer Genome Atlas (TCGA) and normal control from Genotype-Tissue Expression (GTEx). DElncRNA-microRNA (miRNA) and miRNA-DEmRNA pairs were predicted using online databases. Then, a competing endogenous RNA (ceRNA) network was constructed. Functional enrichment analysis of DEmRNAs in the ceRNA network was performed. Protein-protein interaction (PPI) network was then constructed. Hub genes were identified. DElncRNAs in the ceRNA network were validated using Real-time qPCR.
UNASSIGNED: A total of 2,903 up- and 3,228 downregulated mRNAs and 469 up- and 286 downregulated lncRNAs were identified for ALL. A ceRNA network was constructed for ALL, consisting of 845 lncRNA-miRNA and 395 miRNA-mRNA pairs. These DEmRNAs in the ceRNA network were mainly enriched in ALL-related biological processes and pathways. Ten hub genes were identified, including SMAD3, SMAD7, SMAD5, ZFYVE9, FKBP1A, FZD6, FZD7, LRP6, WNT1, and SFRP1. According to Real-time qPCR, eight lncRNAs including ATP11A-AS1, ITPK1-AS1, ANO1-AS2, CRNDE, MALAT1, CACNA1C-IT3, PWRN1, and WT1-AS were significantly upregulated in ALL bone marrow samples compared to normal samples.
UNASSIGNED: Our results showed the lncRNA expression profiles and constructed ceRNA network in ALL. Furthermore, eight lncRNAs including ATP11A-AS1, ITPK1-AS1, ANO1-AS2, CRNDE, MALAT1, CACNA1C-IT3, PWRN1, and WT1-AS were identified. These results could provide a novel insight into the study of ALL.
UNASSIGNED: Differentially expressed mRNAs (DEmRNAs) and differentially expressed long non-coding RNAs (DElncRNAs) were identified for ALL from The Cancer Genome Atlas (TCGA) and normal control from Genotype-Tissue Expression (GTEx). DElncRNA-microRNA (miRNA) and miRNA-DEmRNA pairs were predicted using online databases. Then, a competing endogenous RNA (ceRNA) network was constructed. Functional enrichment analysis of DEmRNAs in the ceRNA network was performed. Protein-protein interaction (PPI) network was then constructed. Hub genes were identified. DElncRNAs in the ceRNA network were validated using Real-time qPCR.
UNASSIGNED: A total of 2,903 up- and 3,228 downregulated mRNAs and 469 up- and 286 downregulated lncRNAs were identified for ALL. A ceRNA network was constructed for ALL, consisting of 845 lncRNA-miRNA and 395 miRNA-mRNA pairs. These DEmRNAs in the ceRNA network were mainly enriched in ALL-related biological processes and pathways. Ten hub genes were identified, including SMAD3, SMAD7, SMAD5, ZFYVE9, FKBP1A, FZD6, FZD7, LRP6, WNT1, and SFRP1. According to Real-time qPCR, eight lncRNAs including ATP11A-AS1, ITPK1-AS1, ANO1-AS2, CRNDE, MALAT1, CACNA1C-IT3, PWRN1, and WT1-AS were significantly upregulated in ALL bone marrow samples compared to normal samples.
UNASSIGNED: Our results showed the lncRNA expression profiles and constructed ceRNA network in ALL. Furthermore, eight lncRNAs including ATP11A-AS1, ITPK1-AS1, ANO1-AS2, CRNDE, MALAT1, CACNA1C-IT3, PWRN1, and WT1-AS were identified. These results could provide a novel insight into the study of ALL.
摘要：
急性淋巴细胞白血病（ALL）是一种最常见于青少年和年轻人的恶性疾病。本研究旨在探索ALL的潜在特征及其功能。
对于来自癌症基因组图谱(TCGA)的ALL和来自基因型-组织表达(GTEx)的正常对照,鉴定了差异表达的mRNA(DEmRNA)和差异表达的长链非编码RNA(DEncRNA)。使用在线数据库预测DElncRNA-微小RNA(miRNA)和miRNA-DEmRNA对。然后，构建了竞争性内源RNA(ceRNA)网络。对ceRNA网络中的DEmRNA进行功能富集分析。然后构建蛋白质-蛋白质相互作用(PPI)网络。鉴定了Hub基因。使用实时qPCR验证ceRNA网络中的DElncRNA。
■对于ALL,鉴定了总共2,903个上调的mRNA和3,228个下调的mRNA以及469个上调的和286个下调的lncRNA。为ALL构建了一个ceRNA网络，由845个lncRNA-miRNA和395个miRNA-mRNA对组成。ceRNA网络中的这些DEmRNA主要富集在ALL相关的生物过程和途径中。确定了十个hub基因，包括SMAD3，SMAD7，SMAD5，ZFYVE9，FKBP1A，FZD6、FZD7、LRP6、WNT1和SFRP1。根据实时qPCR，八个lncRNAs，包括ATP11A-AS1、ITPK1-AS1、ANO1-AS2、CRNDE、与正常样品相比,MALAT1、CACNA1C-IT3、PWRN1和WT1-AS在所有骨髓样品中显著上调。
■我们的结果显示了ALL中的lncRNA表达谱和构建的ceRNA网络。此外，八个lncRNAs，包括ATP11A-AS1、ITPK1-AS1、ANO1-AS2、CRNDE、鉴定了MALAT1、CACNA1C-IT3、PWRN1和WT1-AS。这些结果可以为ALL的研究提供新的见解。
对于来自癌症基因组图谱(TCGA)的ALL和来自基因型-组织表达(GTEx)的正常对照,鉴定了差异表达的mRNA(DEmRNA)和差异表达的长链非编码RNA(DEncRNA)。使用在线数据库预测DElncRNA-微小RNA(miRNA)和miRNA-DEmRNA对。然后，构建了竞争性内源RNA(ceRNA)网络。对ceRNA网络中的DEmRNA进行功能富集分析。然后构建蛋白质-蛋白质相互作用(PPI)网络。鉴定了Hub基因。使用实时qPCR验证ceRNA网络中的DElncRNA。
■对于ALL,鉴定了总共2,903个上调的mRNA和3,228个下调的mRNA以及469个上调的和286个下调的lncRNA。为ALL构建了一个ceRNA网络，由845个lncRNA-miRNA和395个miRNA-mRNA对组成。ceRNA网络中的这些DEmRNA主要富集在ALL相关的生物过程和途径中。确定了十个hub基因，包括SMAD3，SMAD7，SMAD5，ZFYVE9，FKBP1A，FZD6、FZD7、LRP6、WNT1和SFRP1。根据实时qPCR，八个lncRNAs，包括ATP11A-AS1、ITPK1-AS1、ANO1-AS2、CRNDE、与正常样品相比,MALAT1、CACNA1C-IT3、PWRN1和WT1-AS在所有骨髓样品中显著上调。
■我们的结果显示了ALL中的lncRNA表达谱和构建的ceRNA网络。此外，八个lncRNAs，包括ATP11A-AS1、ITPK1-AS1、ANO1-AS2、CRNDE、鉴定了MALAT1、CACNA1C-IT3、PWRN1和WT1-AS。这些结果可以为ALL的研究提供新的见解。
