关键词: Hard Medium-hard and soft indian wheat Murashige and skoog medium Photoperiod Speed-breeding Wheatgrass

来  源:   DOI:10.1007/s13197-020-04805-8   PDF(Sci-hub)   PDF(Pubmed)

Abstract:
The effect of photoperiod durations (16 h light:8 h dark vs 22 h light:2 h dark) and different doses (0.5x and 1x) of Murashige and Skoog medium on the yield and antioxidant characteristics of wheatgrass from hard, medium-hard and soft wheat varieties were analyzed. The average wheatgrass height and wheatgrass yield increased in MS media both under normal photoperiod as well as in water under prolonged photoperiod. An increase in total phenolic content (TPC) and ferric reducing antioxidant power (FRAP) of wheatgrass in different strengths of MS media under normal photoperiod was observed. Whereas, increase in protein content, chlorophyll (Chl) a, Chl b, total Chl, average TPC, DPPH inhibition and FRAP values were observed for wheatgrass of different varieties grown in water under prolonged photoperiod. The accumulation of polypeptides (PPs) of 92 kDa, 33 kDa, 23 kDa, 14 kDa, 12 kDa, and 10 kDa for wheatgrass shoot powder of different varieties was affected by strength of MS media and duration of photoperiod. On the contrary, wheatgrass juice powder showed major changes in the accumulation of PPs 33 kDa and 23 kDa PPs under varied strength of MS media and prolonged photoperiod.
摘要:
光周期持续时间(16h光照:8h黑暗与22h光照:2h黑暗)和不同剂量(0.5x和1x)的Murashige和Skoog培养基对硬草的产量和抗氧化特性的影响,对中硬小麦和软小麦品种进行了分析。在正常光周期下以及在延长光周期下,MS培养基中的平均麦草高度和麦草产量均增加。在正常光周期下,在不同强度的MS培养基中,麦草的总酚含量(TPC)和三价铁还原抗氧化能力(FRAP)均增加。然而,蛋白质含量增加,叶绿素(Chl)a,Chlb,总Chl,平均TPC,在延长的光周期下,观察到在水中生长的不同品种的小麦草的DPPH抑制和FRAP值。92kDa的多肽(PPs)的积累,33kDa,23kDa,14kDa,12kDa,不同品种的麦草枝粉和10kDa受MS培养基强度和光周期持续时间的影响。相反,在不同强度的MS培养基和延长的光周期下,小麦草汁粉显示出PPs33kDa和23kDaPPs的积累发生了重大变化。
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