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Abstract：
Oral fluid is a valuable alternative matrix for forensic toxicologists due to ease of observed collection, limited biohazardous exposure, and indications of recent drug use. Limited information is available for fentanyl analog prevalence, interpretation, or analysis in oral fluid. With increasing numbers of fentanyl-related driving under the influence of drug (DUID) cases appearing in the United States, the development of detection methods is critical. The purpose of the present study was to develop and validate a quantitative method for fentanyl analogs in oral fluid (collected via Quantisal™) using liquid chromatography-quadrupole-time-of-flight-mass spectrometry (LC-QTOF-MS). Validation resulted in limits of detection and quantification ranging from 0.5 to 1 ng/mL. Established linear range was 1-100 ng/mL for all analytes, except acetyl fentanyl at 0.5-100 ng/mL (R2 > 0.994). Within- and between-run precision and bias were considered acceptable with maximum values of ±15.2%CV and ±14.1%, respectively. Matrix effects exhibited ionization enhancement for all analytes with intensified enhancement at a low concentration (9.3-47.4%). No interferences or carryover was observed. Fentanyl analogs were stable in processed extracts stored in the autosampler (4⁰ C) for 48h. The validated method was used to quantify fentanyl analogs in authentic oral fluid samples (n=17) from probationers/parolees. Fentanyl and 4-ANPP concentrations were 1.0-104.5 ng/mL and 1.2-5.7 ng/mL, respectively.
摘要：
由于易于观察收集，口服液是法医毒理学家的有价值的替代基质，有限的生物危险暴露，以及最近吸毒的迹象。芬太尼类似物患病率的信息有限，解释,或口腔液分析。随着在美国出现的与芬太尼相关的药物（DUID）影响下的驾驶病例越来越多，检测方法的发展至关重要。本研究的目的是使用液相色谱-四极杆-飞行时间-质谱（LC-QTOF-MS）开发和验证口服液中芬太尼类似物的定量方法（通过Quantisal™收集）。验证导致检测和定量的极限为0.5至1ng/mL。所有分析物的线性范围为1-100ng/mL，除了乙酰芬太尼在0.5-100ng/mL（R2>0.994）。在±15.2%CV和±14.1%的最大值下，运行中和运行间的精度和偏差被认为是可以接受的，分别。基体效应对所有分析物都表现出电离增强，在低浓度（9.3-47.4％）时增强。没有观察到干扰或残留。芬太尼类似物在处理过的提取物中稳定储存在自动进样器(4°C)中48小时。经验证的方法用于量化来自见证者/假释者的真实口腔液样品(n=17)中的芬太尼类似物。芬太尼和4-ANPP浓度分别为1.0-104.5ng/mL和1.2-5.7ng/mL，分别。
