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Abstract：
UNASSIGNED: This study aimed to construct drug-loading and drug-releasing quantitative equations for gentamicin sponges in addition to realizing a gentamicin sponge for wound infection prevention and treatment.
UNASSIGNED: Sterile sponges were cut into pieces of 1×1 × 0.5 cm and immersed in 40, 16, 8, 4, 1.6, 0.8, or 0 mg/mL of gentamicin solution for 12, 24, 48, 96, or 120 h to evaluate their gentamicin loading. The sponges were subsequently immersed in the gentamicin solution of different concentrations for 48 h, air-dried, and then immersed in 10 mL of 0.9% physiological saline to evaluate the gentamicin release. Methicillin-sensitive Staphylococcus aureus (MSSA) and Pseudomonas aeruginosa were used to explore the sponges\' infection prevention scheme. In addition, a rat femur fracture with wound infection model was used to assess the infection treatment scheme.
UNASSIGNED: The antibacterial zone sizes of the sponges immersed in 40, 16, 8, 4, 1.6, and 0.8 mg/mL of the gentamicin solution were larger than those of the 0 mg/mL air-dried sponge, and the difference was statistically significant (p < 0.01, p < 0.01, p < 0.01, p < 0.01, p < 0.01, and p < 0.01, respectively). The rats in the 40, 16, and 8 mg/mL air-dried sponge groups had no wound suppuration in either the MSSA or P. aeruginosa rat infection models.
UNASSIGNED: A quantified equation for the sponges\' gentamicin loading and release was achieved with high accuracy. Furthermore, we recommend the 40, 16, or 8 mg/mL air-dried sponge for the treatment of wounds with antibiotic-sensitive bacterial infections.
UNASSIGNED: Sterile sponges were cut into pieces of 1×1 × 0.5 cm and immersed in 40, 16, 8, 4, 1.6, 0.8, or 0 mg/mL of gentamicin solution for 12, 24, 48, 96, or 120 h to evaluate their gentamicin loading. The sponges were subsequently immersed in the gentamicin solution of different concentrations for 48 h, air-dried, and then immersed in 10 mL of 0.9% physiological saline to evaluate the gentamicin release. Methicillin-sensitive Staphylococcus aureus (MSSA) and Pseudomonas aeruginosa were used to explore the sponges\' infection prevention scheme. In addition, a rat femur fracture with wound infection model was used to assess the infection treatment scheme.
UNASSIGNED: The antibacterial zone sizes of the sponges immersed in 40, 16, 8, 4, 1.6, and 0.8 mg/mL of the gentamicin solution were larger than those of the 0 mg/mL air-dried sponge, and the difference was statistically significant (p < 0.01, p < 0.01, p < 0.01, p < 0.01, p < 0.01, and p < 0.01, respectively). The rats in the 40, 16, and 8 mg/mL air-dried sponge groups had no wound suppuration in either the MSSA or P. aeruginosa rat infection models.
UNASSIGNED: A quantified equation for the sponges\' gentamicin loading and release was achieved with high accuracy. Furthermore, we recommend the 40, 16, or 8 mg/mL air-dried sponge for the treatment of wounds with antibiotic-sensitive bacterial infections.
摘要：
■本研究旨在构建庆大霉素海绵的载药量和释药定量方程,并实现一种用于伤口感染预防和治疗的庆大霉素海绵。
■将无菌海绵切成1×1×0.5cm的片，并浸入40、16、8、4、1.6、0.8或0mg/mL的庆大霉素溶液中12、24、48、96或120小时，以评估其庆大霉素负载。随后将海绵浸入不同浓度的庆大霉素溶液中48小时，风干,然后浸入10mL的0.9％生理盐水中，以评估庆大霉素的释放。使用甲氧西林敏感金黄色葡萄球菌（MSSA）和铜绿假单胞菌探索海绵感染预防方案。此外,使用大鼠股骨骨折伴伤口感染模型评估感染治疗方案。
■浸入40、16、8、4、1.6和0.8mg/mL庆大霉素溶液中的海绵的抗菌区尺寸大于0mg/mL风干海绵的抗菌区尺寸，且差异有统计学意义(分别为p<0.01、p<0.01、p<0.01、p<0.01、p<0.01、p<0.01)。在MSSA或铜绿假单胞菌大鼠感染模型中，40、16和8mg/mL风干海绵组中的大鼠没有伤口化脓。
■以高精度实现了海绵庆大霉素负载和释放的定量方程。此外，我们推荐使用40,16或8mg/mL风干海绵治疗抗生素敏感细菌感染伤口.
■将无菌海绵切成1×1×0.5cm的片，并浸入40、16、8、4、1.6、0.8或0mg/mL的庆大霉素溶液中12、24、48、96或120小时，以评估其庆大霉素负载。随后将海绵浸入不同浓度的庆大霉素溶液中48小时，风干,然后浸入10mL的0.9％生理盐水中，以评估庆大霉素的释放。使用甲氧西林敏感金黄色葡萄球菌（MSSA）和铜绿假单胞菌探索海绵感染预防方案。此外,使用大鼠股骨骨折伴伤口感染模型评估感染治疗方案。
■浸入40、16、8、4、1.6和0.8mg/mL庆大霉素溶液中的海绵的抗菌区尺寸大于0mg/mL风干海绵的抗菌区尺寸，且差异有统计学意义(分别为p<0.01、p<0.01、p<0.01、p<0.01、p<0.01、p<0.01)。在MSSA或铜绿假单胞菌大鼠感染模型中，40、16和8mg/mL风干海绵组中的大鼠没有伤口化脓。
■以高精度实现了海绵庆大霉素负载和释放的定量方程。此外，我们推荐使用40,16或8mg/mL风干海绵治疗抗生素敏感细菌感染伤口.
