RESULTS: Proteomic analysis indicated that 111 proteins were differentially expressed in the liver of broiler chickens from the immune stress group. Of these, 28 proteins were down-regulated, and 83 proteins were up-regulated in the immune stress group. Enrichment analysis showed that immune stress upregulated the expression of hepatic proteins involved in defense function, amino acid catabolism, ion transport, wound healing, and hormone secretion. Furthermore, immune stress increased valine, leucine and isoleucine degradation pathways.
CONCLUSIONS: The data suggests that growth depression of broiler chickens induced by immune stress is triggered by hepatic proteome alterations, and provides a new insight into the mechanism by which immune challenge impairs poultry production.
结果:蛋白质组学分析表明,免疫应激组肉鸡肝脏中有111种蛋白质差异表达。其中,28种蛋白质被下调,免疫应激组中有83种蛋白上调。富集分析显示,免疫应激上调肝脏防御功能相关蛋白的表达,氨基酸分解代谢,离子传输,伤口愈合,和激素分泌。此外,免疫应激增加缬氨酸,亮氨酸和异亮氨酸降解途径。
结论:数据表明,免疫应激诱导的肉鸡生长抑制是由肝脏蛋白质组改变引发的,并为免疫挑战损害家禽生产的机制提供了新的见解。