METHODS: This study included 63 subjects comprising of 25 OL, 26 OSCC, and 12 OPSCC cases. Exfoliated cells were collected and processed for PCR followed by Papanicolaou staining and subsequent grading. Additionally the non-classical signs were evaluated and statistical analysis included Chi-square and Spearman\'s test.
RESULTS: 23/63 (36.5%) cases showed PCR positivity for HPV16. Most of the cytomorphological features showed significant correlation for the presence of HPV. A greater sensitivity and specificity was observed in the Bethesda system for reporting cervical cytology (TBS) than the Papanicolaou grading system.
CONCLUSIONS: We conclude that the non-classic cytological features could be employed in the detection of HPV in low resource settings with improved sensitivity. Liquid based cytology graded using TBS could be suitable for oral cytology in the detection of early atypical changes.
方法:这项研究包括63名受试者,包括25名OL,26OSCC,和12个OPSCC病例。收集脱落的细胞并处理用于PCR,然后进行巴氏染色和随后的分级。此外,还评估了非经典体征,统计分析包括卡方和Spearman检验。
结果:23/63(36.5%)例HPV16PCR阳性。大多数细胞形态学特征显示与HPV的存在显着相关性。与Papanicolaou分级系统相比,Bethesda系统报告宫颈细胞学(TBS)的敏感性和特异性更高。
结论:我们得出结论,非经典细胞学特征可用于低资源环境中的HPV检测,并具有更高的灵敏度。使用TBS进行液基细胞学分级可适用于口腔细胞学中早期非典型改变的检测。