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Abstract：
Mid-infrared (MIR) microscopic imaging of indolent and aggressive lymphomas was performed including formalin-fixed and paraffin-embedded samples of six follicular lymphomas and 12 diffuse large B-cell-lymphomas as well as reactive lymph nodes to investigate benefits and challenges for lymphoma diagnosis. MIR images were compared to defined pathological characteristics such as indolent versus aggressive versus reactive, germinal centre versus activated cell-of-origin (COO) subtypes, or a low versus a high proliferative index and level of PD-L1 expression. We demonstrated that MIR microscopic imaging can differentiate between reactive lymph nodes, indolent and aggressive lymphoma samples. Also, it has potential to be used in the subtyping of lymphomas, as shown with the differentiation between COO subtypes, the level of proliferation and PD-L1 expression. MIR microscopic imaging is a promising tool for diagnosis and subtyping of lymphoma and further evaluation is needed to fully explore the advantages and disadvantages of this method for pathological diagnosis.
摘要：
进行了惰性和侵袭性淋巴瘤的中红外（MIR）显微成像，包括福尔马林固定和石蜡包埋的6个滤泡性淋巴瘤和12个弥漫性大B细胞淋巴瘤以及反应性淋巴结，以研究益处和挑战淋巴瘤诊断。将MIR图像与定义的病理特征进行比较，例如惰性，侵袭性和反应性，生发中心与活化细胞起源（COO）亚型，或低与高增殖指数和PD-L1表达水平。我们证明MIR显微成像可以区分反应性淋巴结，惰性和侵袭性淋巴瘤样本。此外，它有可能用于淋巴瘤的亚型，如COO亚型之间的区别所示，增殖水平和PD-L1表达。MIR显微成像是淋巴瘤诊断和分型的一种有前途的工具，需要进一步评估以充分探索这种方法用于病理诊断的优缺点。
