METHODS: Epulis and normal gingival tissues were collected, and AhR expression was detected at the mRNA and protein levels by quantitative polymerase chain reaction (qPCR) and immunohistochemistry, respectively. The expression levels of proinflammatory cytokines and apoptosis-related factor genes in human periodontal ligament cells (hPDLCs) and human gingival fibroblasts (hGFs) transfected with AhR short interfering RNA (siRNA) or negative control siRNA, upon stimulation with lipopolysaccharide of Porphyromonas gingivalis (Pg-LPS), were then examined. Finally, the expression levels of the proinflammatory cytokines and apoptosis-related factor genes in the epulis tissues were observed by qPCR.
RESULTS: AhR expression in fibrous epulis was significantly increased at both the mRNA and protein levels. The expression of proinflammatory cytokines and apoptosis-related factor genes in hPDLCs transfected with AhR siRNA was significantly decreased when stimulated with Pg-LPS. The same trends were observed for hGFs. The opposite trend was detected in the epulis tissues.
CONCLUSIONS: AhR may be a key factor in fibrous epulis pathogenesis that acts by regulating the expression of BCL2 family genes and inflammatory factor-related genes.
方法:收集牙龈组织和正常牙龈组织,通过定量聚合酶链反应(qPCR)和免疫组织化学在mRNA和蛋白质水平检测AhR表达,分别。转染AhR短干扰RNA(siRNA)或阴性对照siRNA的人牙周膜细胞(hPDLCs)和人牙龈成纤维细胞(hGFs)中促炎细胞因子和凋亡相关因子基因的表达水平,在用牙龈卟啉单胞菌(Pg-LPS)的脂多糖刺激后,然后检查。最后,采用qPCR方法观察各组大鼠表皮组织中促炎细胞因子和凋亡相关因子基因的表达水平。
结果:AhR在纤维性腺中的表达在mRNA和蛋白水平上都显著增加。用Pg-LPS刺激时,AhRsiRNA转染的hPDLCs中促炎细胞因子和凋亡相关因子基因的表达明显降低。对于hGF观察到相同的趋势。在表皮组织中检测到相反的趋势。
结论:AhR可能通过调节BCL2家族基因和炎症因子相关基因的表达而成为纤维性血管形成的关键因素。