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Abstract：
Creating aneurysm sizes in animal models that resemble human aneurysms is essential to study and test neuroendovascular devices. The commonly used rabbit surgical elastase model, however, produces saccular aneurysms that are smaller than those typically treated in humans. The goal of this study was to determine whether an increased vessel stump length and the addition of calcium chloride to the incubation solution has an effect on the resulting aneurysm size.
Using a modified aneurysm creation method, 32 female New Zealand White rabbits underwent aneurysm creation procedures. Subjects were equally allocated into 4 different groups based on vessel stump length (2 cm controls vs. 3 cm) and incubation solution (elastase alone controls vs. a 1:1 mixture of elastase and calcium chloride). At 4 weeks, all animals underwent angiography to determine the resulting aneurysm size by a neurointerventionalist who was blinded to treatment group.
An increase in stump length from 2 cm to 3 cm resulted in a significant increase in the height of aneurysm (P < 0.05). Compared with control animals, the combination of a 3-cm stump length and the addition of calcium chloride to the incubation solution resulted in a significant increase in aneurysm height, width, and volume (P < 0.05).
Creating larger aneurysms is necessary for the rabbit model to be more clinically relevant. Our study demonstrated that the utilization of a 3-cm vessel stump as well as both calcium chloride and elastase in the incubation solution results in aneurysm sizes that more closely resemble the population of aneurysms treated in humans.
Using a modified aneurysm creation method, 32 female New Zealand White rabbits underwent aneurysm creation procedures. Subjects were equally allocated into 4 different groups based on vessel stump length (2 cm controls vs. 3 cm) and incubation solution (elastase alone controls vs. a 1:1 mixture of elastase and calcium chloride). At 4 weeks, all animals underwent angiography to determine the resulting aneurysm size by a neurointerventionalist who was blinded to treatment group.
An increase in stump length from 2 cm to 3 cm resulted in a significant increase in the height of aneurysm (P < 0.05). Compared with control animals, the combination of a 3-cm stump length and the addition of calcium chloride to the incubation solution resulted in a significant increase in aneurysm height, width, and volume (P < 0.05).
Creating larger aneurysms is necessary for the rabbit model to be more clinically relevant. Our study demonstrated that the utilization of a 3-cm vessel stump as well as both calcium chloride and elastase in the incubation solution results in aneurysm sizes that more closely resemble the population of aneurysms treated in humans.
摘要：
在动物模型中创建类似于人类动脉瘤的动脉瘤大小对于研究和测试神经血管内设备至关重要。手术常用的兔弹性蛋白酶模型,然而,产生的囊状动脉瘤比人类通常治疗的小。这项研究的目的是确定增加的血管残端长度和向孵育溶液中添加氯化钙是否对所得的动脉瘤大小有影响。
使用改进的动脉瘤创建方法，32只雌性新西兰白兔接受了动脉瘤创建程序。根据血管残端长度将受试者平均分为4组（2cm对照与3厘米）和孵育溶液（仅弹性蛋白酶对照与弹性蛋白酶和氯化钙的1:1混合物)。4周时,所有动物均接受血管造影检查,由一名神经介入医师确定动脉瘤大小,该医师对治疗组不知情.
残端长度从2cm增加到3cm导致动脉瘤高度显着增加（P<0.05）。与对照动物相比，3厘米的残端长度和向孵育溶液中添加氯化钙的组合导致动脉瘤高度显着增加，宽度,体积(P<0.05)。
创建更大的动脉瘤对于兔模型更具有临床相关性是必要的。我们的研究表明，在孵育溶液中使用3厘米的血管残端以及氯化钙和弹性蛋白酶会导致动脉瘤的大小更类似于人类治疗的动脉瘤。
使用改进的动脉瘤创建方法，32只雌性新西兰白兔接受了动脉瘤创建程序。根据血管残端长度将受试者平均分为4组（2cm对照与3厘米）和孵育溶液（仅弹性蛋白酶对照与弹性蛋白酶和氯化钙的1:1混合物)。4周时,所有动物均接受血管造影检查,由一名神经介入医师确定动脉瘤大小,该医师对治疗组不知情.
残端长度从2cm增加到3cm导致动脉瘤高度显着增加（P<0.05）。与对照动物相比，3厘米的残端长度和向孵育溶液中添加氯化钙的组合导致动脉瘤高度显着增加，宽度,体积(P<0.05)。
创建更大的动脉瘤对于兔模型更具有临床相关性是必要的。我们的研究表明，在孵育溶液中使用3厘米的血管残端以及氯化钙和弹性蛋白酶会导致动脉瘤的大小更类似于人类治疗的动脉瘤。
