Mesh : Animals Diet Food, Genetically Modified Genitalia Mice Plants, Genetically Modified / genetics Polymerase Chain Reaction

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Abstract:
The rapid development of molecular biology and genetic engineering contributes to the creation of plants with desired properties in a short time. One of the aspects of the study of genetically modified organisms (GMO) and genetically modified products (GMP) is the study of their impact on humans, animals and the environment. Subject of research - changes in the morph functional indicators of the reproductive system of mice. The relevance of the chosen topic is due to the importance of the reproductive system for the reproduction of a healthy generation, capable of developing normally and continuing its race. Purpose of the study - to identify the effect of GMOs on the reproductive system of mice. Cultivation of three groups of laboratory mice using transgenic feed (genetically modified soybean meal) and obtaining biological material for research. Determination of the presence of genetically modified sources (GMS), genetically modified organisms (GMO) in food and feed products using the polymerase chain reaction (PCR). Morphometric study of the obtained material and biometric data processing. It has been shown that the consumption of feed by animals prepared on the basis of genetically modified plants does not affect the reproductive functions of the parental generation; but at the same time there was an inhibition of the growth rate and the process of formation of the gonads of the descendants of the first and, especially, the second generation; the second generation of offspring, eating only soybean meal, had defective reproductive qualities and high mortality. Second-generation mice eating genetically modified soybean meal are at greater risk than second-generation mice eating traditional diets.
摘要:
分子生物学和基因工程的快速发展有助于在短时间内创造具有所需特性的植物。研究转基因生物(GMO)和转基因产品(GMP)的一个方面是研究它们对人类的影响,动物和环境。研究主题-小鼠生殖系统形态功能指标的变化。所选主题的相关性是由于生殖系统对于健康一代的生殖的重要性,能够正常发展并继续比赛。研究目的-确定转基因生物对小鼠生殖系统的影响。采用转基因饲料(转基因豆粕)培养三组实验小鼠,并获得生物材料进行研究。确定转基因来源(GMS)的存在,使用聚合酶链反应(PCR)在食品和饲料产品中使用转基因生物(GMO)。获得的材料的形态计量学研究和生物特征数据处理。研究表明,以转基因植物为基础制备的动物食用饲料不会影响亲代的生殖功能;但同时抑制了第一和子代生殖腺的生长速度和形成过程,尤其是,第二代;第二代后代,只吃豆粕,生殖质量有缺陷,死亡率高。吃转基因豆粕的第二代小鼠比吃传统饮食的第二代小鼠面临更大的风险。
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