PDF(Pubmed)
Abstract：
UNASSIGNED: The peste des petits ruminants (PPR) is a highly contagious disease of small ruminants which negatively affects animal production and the socioeconomic status of farmers. Peste des petits ruminants virus (PPRV) encodes eight proteins, with the viral fusion protein (F) playing a role in virus virulence and stimulating an effective protective immune response. This study aimed to isolate and complete the identification of PPRV circulating in goats in different Egyptian governorates and perform molecular characterization of the PPRV F gene.
UNASSIGNED: Samples were collected from unvaccinated animals with clinical signs suggestive of PPR. A total of 256 sera were tested for the detection of PPRV antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA) kit, while 214 samples of blood buffy coat preparation, animal swabs (nasal, ocular, and saliva), and fecal and tissue samples were tested for the detection of the PPRV antigen using an antigen-capture ELISA kit. Molecular diagnosis, gene cloning, blast analysis, and phylogenetic analysis were performed for the molecular characterization of PPRV.
UNASSIGNED: The seroprevalence results of PPRV antibodies in the tested sera showed a total of 67.9% positive samples. The rates of PPR antigen recorded by the antigen-capture ELISA in the swabs (nasal and ocular) and tissue samples were 44.3%, 46.8%, and 43.5%, respectively, with saliva swabs having the highest rate of PPRV positivity (76.4%) and fecal samples having the lowest (33.3%). Molecular characterization of the PPRV Vero cell culture revealed that the circulating PPRV strain belongs to the IV lineage. Blast analysis of the PPRV F gene showed 96.7% identity with the PPRV strain Egypt-2014 fusion protein (F) gene, KT006589.1, differing by 43 single-nucleotide polymorphisms.
UNASSIGNED: The results of this study indicate that the emerging PPRV belongs to the IV lineage among small ruminant animals. The findings also indicate the need for an innovative strategy to control and eliminate this disease based on a regularly administered and effective vaccine, a test to distinguish between infected and vaccinated animals, and the need for further study on the protein structure and PPRV F gene expression, which should help us to understand the molecular evolution of the virus and control and eliminate PPR disease.
UNASSIGNED: Samples were collected from unvaccinated animals with clinical signs suggestive of PPR. A total of 256 sera were tested for the detection of PPRV antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA) kit, while 214 samples of blood buffy coat preparation, animal swabs (nasal, ocular, and saliva), and fecal and tissue samples were tested for the detection of the PPRV antigen using an antigen-capture ELISA kit. Molecular diagnosis, gene cloning, blast analysis, and phylogenetic analysis were performed for the molecular characterization of PPRV.
UNASSIGNED: The seroprevalence results of PPRV antibodies in the tested sera showed a total of 67.9% positive samples. The rates of PPR antigen recorded by the antigen-capture ELISA in the swabs (nasal and ocular) and tissue samples were 44.3%, 46.8%, and 43.5%, respectively, with saliva swabs having the highest rate of PPRV positivity (76.4%) and fecal samples having the lowest (33.3%). Molecular characterization of the PPRV Vero cell culture revealed that the circulating PPRV strain belongs to the IV lineage. Blast analysis of the PPRV F gene showed 96.7% identity with the PPRV strain Egypt-2014 fusion protein (F) gene, KT006589.1, differing by 43 single-nucleotide polymorphisms.
UNASSIGNED: The results of this study indicate that the emerging PPRV belongs to the IV lineage among small ruminant animals. The findings also indicate the need for an innovative strategy to control and eliminate this disease based on a regularly administered and effective vaccine, a test to distinguish between infected and vaccinated animals, and the need for further study on the protein structure and PPRV F gene expression, which should help us to understand the molecular evolution of the virus and control and eliminate PPR disease.
摘要：
■小反刍动物（PPR）是小反刍动物的高度传染性疾病，对动物生产和农民的社会经济地位产生负面影响。小反刍动物病毒（PPRV）编码八种蛋白质，病毒融合蛋白（F）在病毒毒力和刺激有效的保护性免疫应答中起作用。本研究旨在分离和完成在埃及不同省份山羊中循环的PPRV的鉴定，并对PPRVF基因进行分子表征。
从具有提示PPR的临床体征的未接种动物收集样品。用竞争性酶联免疫吸附试验(c-ELISA)试剂盒检测PPRV抗体共256份血清,而214个血沉棕黄层样本的制备，动物拭子（鼻，眼,和唾液)，使用抗原捕获ELISA试剂盒测试粪便和组织样品对PPRV抗原的检测。分子诊断,基因克隆,爆炸分析,并对PPRV的分子特征进行了系统发育分析。
■测试血清中PPRV抗体的血清阳性率结果显示总共67.9％的阳性样品。通过抗原捕获ELISA在拭子（鼻和眼）和组织样本中记录的PPR抗原率为44.3％，46.8%,和43.5%，分别,唾液拭子的PPRV阳性率最高（76.4％），粪便样本最低（33.3％）。PPRVVero细胞培养物的分子表征显示循环的PPRV菌株属于IV谱系。对PPRVF基因的Blast分析显示与PPRV菌株埃及-2014融合蛋白（F）基因具有96.7％的同一性，KT006589.1，差异有43个单核苷酸多态性。
■这项研究的结果表明，新兴的PPRV属于小反刍动物中的IV谱系。研究结果还表明，需要一种基于定期施用和有效疫苗的创新策略来控制和消除这种疾病，区分感染和接种疫苗的动物的测试，蛋白结构和PPRVF基因表达需要进一步研究,这应该有助于我们了解病毒的分子进化，控制和消除PPR疾病。
从具有提示PPR的临床体征的未接种动物收集样品。用竞争性酶联免疫吸附试验(c-ELISA)试剂盒检测PPRV抗体共256份血清,而214个血沉棕黄层样本的制备，动物拭子（鼻，眼,和唾液)，使用抗原捕获ELISA试剂盒测试粪便和组织样品对PPRV抗原的检测。分子诊断,基因克隆,爆炸分析,并对PPRV的分子特征进行了系统发育分析。
■测试血清中PPRV抗体的血清阳性率结果显示总共67.9％的阳性样品。通过抗原捕获ELISA在拭子（鼻和眼）和组织样本中记录的PPR抗原率为44.3％，46.8%,和43.5%，分别,唾液拭子的PPRV阳性率最高（76.4％），粪便样本最低（33.3％）。PPRVVero细胞培养物的分子表征显示循环的PPRV菌株属于IV谱系。对PPRVF基因的Blast分析显示与PPRV菌株埃及-2014融合蛋白（F）基因具有96.7％的同一性，KT006589.1，差异有43个单核苷酸多态性。
■这项研究的结果表明，新兴的PPRV属于小反刍动物中的IV谱系。研究结果还表明，需要一种基于定期施用和有效疫苗的创新策略来控制和消除这种疾病，区分感染和接种疫苗的动物的测试，蛋白结构和PPRVF基因表达需要进一步研究,这应该有助于我们了解病毒的分子进化，控制和消除PPR疾病。
