PDF(Sci-hub)
Abstract：
Lumpy skin disease (LSD), an economically significant disease in cattle caused by lumpy skin disease virus (LSDV), is endemic to nearly all of Africa. Since 2012, LSDV has emerged as a significant epizootic pathogen given its rapid spread into new geographical locations outside Africa, including the Middle East, Eastern Europe, and Asia. To assess the genetic diversity of LSDVs in East Africa, we sequenced and analyzed the RPO30 and GPCR genes of LSDV in twenty-two archive samples collected in Ethiopia, Kenya, and Sudan before the appearance of LSD in the Middle East and its incursion into Europe. We compared them to publicly available sequences of LSDVs from the same region and those collected elsewhere. The results showed that the East African field isolates in this study were remarkably similar to each other and to previously sequenced field isolates of LSDV for the RPO30 and GPCR genes. The only exception was LSDV Embu/B338/2011, a field virus collected in Kenya, which displayed mixed features between the LSDV Neethling vaccine and field isolates. LSDV Embu/B338/2011 had the same 12-nucleotide insertion found in LSDV Neethling and KS-1 vaccines. Further analysis of the partial EEV glycoprotein, B22R, RNA helicase, virion core protein, NTPase, and N1R/p28-like protein genes showed that LSDV Embu/B338/2011 differs from previously described LSDV variants carrying the 12-nucleotide insertion in the GPCR gene. These findings highlight the importance of the constant monitoring of genetic variation among LSDV isolates.
摘要：
块状皮肤病（LSD），由块状皮肤病病毒（LSDV）引起的牛的经济上重要的疾病，几乎是整个非洲的地方病。自2012年以来，LSDV已成为一种重要的流行病病原体，因为它迅速传播到非洲以外的新地理位置，包括中东,东欧,和亚洲。为了评估东非LSDV的遗传多样性，我们在埃塞俄比亚收集的22个档案样本中对LSDV的RPO30和GPCR基因进行了测序和分析，肯尼亚,在LSD在中东出现并入侵欧洲之前，还有苏丹。我们将它们与来自同一地区的公开可用的LSDV序列以及其他地方收集的序列进行了比较。结果表明，本研究中的东非田间分离株彼此非常相似，并且与先前测序的LSDV的RPO30和GPCR基因的田间分离株非常相似。唯一的例外是LSDVEmbu/B338/2011，一种在肯尼亚收集的野外病毒，这显示了LSDVNeethling疫苗和现场分离株之间的混合特征。LSDVEmbu/B338/2011具有在LSDVNeethling和KS-1疫苗中发现的相同的12核苷酸插入。部分EEV糖蛋白的进一步分析，B22R,RNA解旋酶,病毒体核心蛋白，NTPase,和N1R/p28样蛋白基因显示LSDVEmbu/B338/2011不同于先前描述的在GPCR基因中携带12个核苷酸插入的LSDV变体。这些发现强调了持续监测LSDV分离株之间遗传变异的重要性。
