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Abstract：
BACKGROUND: Here, we describe an integrative method to detect carbapenemase-producing Gram-negative bacteria (gn-Cp) on surfaces/fomites in the patient environment. We examined environmental samples from 28 patient rooms occupied with patients who were proven to be colonised with gn-Cp by rectal screening.
METHODS: We took samples after 24 h, 72 h and one week. For sampling, we divided the patient environment into four parts and took samples from near- and extended patient areas. To obtain a representative bacterial swab from a larger surface, such as the patient cabinet, we used Polywipes. Bacterial DNA was isolated. Carbapenemase was detected with specific qPCR primers.
RESULTS: With this culture- and molecular-based approach, we could control the effectiveness of cleaning and disinfection in everyday clinical practice. Therefore, we could track the spread of gn-Cp within the patient room. The number of positive detections fluctuated between 30.5% (mean value positive results after 72 h) and 35.2% (after 24 h and one week).
CONCLUSIONS: The method used to detect multidrug-resistant bacteria in the environment of patients by using PolywipesTM is reliable and can therefore be used as an effective, new tool in hygiene and infection control.
METHODS: We took samples after 24 h, 72 h and one week. For sampling, we divided the patient environment into four parts and took samples from near- and extended patient areas. To obtain a representative bacterial swab from a larger surface, such as the patient cabinet, we used Polywipes. Bacterial DNA was isolated. Carbapenemase was detected with specific qPCR primers.
RESULTS: With this culture- and molecular-based approach, we could control the effectiveness of cleaning and disinfection in everyday clinical practice. Therefore, we could track the spread of gn-Cp within the patient room. The number of positive detections fluctuated between 30.5% (mean value positive results after 72 h) and 35.2% (after 24 h and one week).
CONCLUSIONS: The method used to detect multidrug-resistant bacteria in the environment of patients by using PolywipesTM is reliable and can therefore be used as an effective, new tool in hygiene and infection control.
摘要：
背景：这里，我们描述了一种检测患者环境中表面/fomites上产生碳青霉烯酶的革兰氏阴性菌（gn-Cp）的综合方法。我们检查了来自28个病房的环境样本，这些患者被直肠筛查证实被gn-Cp定植。
方法：我们在24小时后取样，72小时和一周。对于采样，我们将患者环境分为四个部分，并从邻近和延伸的患者区域采集样本。为了从更大的表面获得代表性的细菌拭子，比如病人柜，我们用了Polywipes.分离细菌DNA。用特异性qPCR引物检测碳青霉烯酶。
结果：通过这种基于文化和分子的方法，我们可以在日常临床实践中控制清洁和消毒的有效性。因此,我们可以追踪gn-Cp在病房内的传播。阳性检测的数量在30.5％（72小时后的平均值阳性结果）和35.2％（24小时和一周后）之间波动。
结论：使用PolywipesTM检测患者环境中多药耐药菌的方法是可靠的，因此可以作为一种有效的，卫生和感染控制的新工具。
方法：我们在24小时后取样，72小时和一周。对于采样，我们将患者环境分为四个部分，并从邻近和延伸的患者区域采集样本。为了从更大的表面获得代表性的细菌拭子，比如病人柜，我们用了Polywipes.分离细菌DNA。用特异性qPCR引物检测碳青霉烯酶。
结果：通过这种基于文化和分子的方法，我们可以在日常临床实践中控制清洁和消毒的有效性。因此,我们可以追踪gn-Cp在病房内的传播。阳性检测的数量在30.5％（72小时后的平均值阳性结果）和35.2％（24小时和一周后）之间波动。
结论：使用PolywipesTM检测患者环境中多药耐药菌的方法是可靠的，因此可以作为一种有效的，卫生和感染控制的新工具。
