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Abstract：
Since human infections with Campylobacter jejuni including antibiotic-resistant strains are rising worldwide, natural compounds might constitute promising antibiotics-independent treatment options for campylobacteriosis. Since the health-beneficial properties of garlic have been known for centuries, we here surveyed the antimicrobial and immune-modulatory effects of garlic essential oil (EO) in acute experimental campylobacteriosis. Therefore, secondary abiotic IL-10-/- mice were orally infected with C. jejuni strain 81-176 and garlic-EO treatment via the drinking water was initiated on day 2 post-infection. Mice from the garlic-EO group displayed less severe clinical signs of acute campylobacteriosis as compared to placebo counterparts that were associated with lower ileal C. jejuni burdens on day 6 post-infection. Furthermore, when compared to placebo application, garlic-EO treatment resulted in alleviated colonic epithelia cell apoptosis, in less pronounced C. jejuni induced immune cell responses in the large intestines, in dampened pro-inflammatory mediator secretion in intestinal and extra-intestinal compartments, and, finally, in less frequent translocation of viable pathogens from the intestines to distinct organs. Given its potent immune-modulatory and disease-alleviating effects as shown in our actual preclinical placebo-controlled intervention study, we conclude that garlic-EO may be considered as promising adjunct treatment option for acute campylobacteriosis in humans.
摘要：
由于人类感染空肠弯曲杆菌包括抗生素耐药菌株在全球范围内呈上升趋势，天然化合物可能构成弯曲杆菌病的有希望的不依赖抗生素的治疗方案.由于大蒜对健康有益的特性已经知道了几个世纪，我们在这里调查了大蒜精油（EO）在急性实验性弯曲杆菌病中的抗菌和免疫调节作用。因此,用空肠弯曲杆菌菌株81-176口服感染继发性非生物IL-10-/-小鼠，并在感染后第2天开始通过饮用水进行大蒜-EO治疗。与在感染后第6天与较低的回肠空肠杆菌负担相关的安慰剂对应物相比,来自大蒜-EO组的小鼠显示出更不严重的急性弯曲杆菌病的临床体征。此外，与安慰剂应用相比，大蒜-EO处理导致结肠上皮细胞凋亡减轻，在不太明显的空肠弯曲杆菌诱导的大肠免疫细胞反应，在抑制肠道和肠外隔室的促炎介质分泌中，and,最后,从肠到不同器官的活病原体转移频率较低。鉴于其有效的免疫调节和疾病缓解作用，如我们实际的临床前安慰剂对照干预研究所示，我们得出的结论是，大蒜-EO可能被认为是人类急性弯曲杆菌病的有希望的辅助治疗选择。
