PDF(Sci-hub)
Abstract：
Gene expression is one of the main factors to influence meat quality by modulating fatty acid metabolism, composition, and deposition rates in muscle tissue. This study aimed to explore the transcriptomics of the Longissimus lumborum muscle in two local pig breeds with distinct genetic background using next-generation sequencing technology and Real-Time qPCR. RNA-seq yielded 49 differentially expressed genes between breeds, 34 overexpressed in the Alentejano (AL) and 15 in the Bísaro (BI) breed. Specific slow type myosin heavy chain components were associated with AL (MYH7) and BI (MYH3) pigs, while an overexpression of MAP3K14 in AL may be associated with their lower loin proportion, induced insulin resistance, and increased inflammatory response via NFkB activation. Overexpression of RUFY1 in AL pigs may explain the higher intramuscular (IMF) content via higher GLUT4 recruitment and consequently higher glucose uptake that can be stored as fat. Several candidate genes for lipid metabolism, excluded in the RNA-seq analysis due to low counts, such as ACLY, ADIPOQ, ELOVL6, LEP and ME1 were identified by qPCR as main gene factors defining the processes that influence meat composition and quality. These results agree with the fatter profile of the AL pig breed and adiponectin resistance can be postulated as responsible for the overexpression of MAP3K14\'s coding product NIK, failing to restore insulin sensitivity.
摘要：
基因表达调控脂肪酸代谢是影响肉品质的主要因素之一。composition,和在肌肉组织中的沉积速率。本研究旨在利用下一代测序技术和实时qPCR技术，探索具有不同遗传背景的两个地方猪品种腰背最长肌的转录组学。RNA-seq在品种之间产生了49个差异表达的基因，在Alentejano（AL）中过表达34，在Bísaro（BI）品种中过表达15。特定的慢型肌球蛋白重链组分与AL（MYH7）和BI（MYH3）猪相关，而AL中MAP3K14的过度表达可能与它们较低的腰部比例有关，诱导胰岛素抵抗，和通过NFkB激活增加的炎症反应。RUFY1在AL猪中的过表达可以通过更高的GLUT4募集来解释更高的肌内（IMF）含量，从而可以作为脂肪储存的更高的葡萄糖摄取。脂质代谢的几个候选基因，由于低计数而排除在RNA-seq分析中，例如,ADIPOQ,通过qPCR将ELOVL6，LEP和ME1鉴定为定义影响肉组成和质量的过程的主要基因因子。这些结果与AL猪品种的脂肪分布一致，脂联素抗性可以被认为是MAP3K14编码产物NIK过度表达的原因。未能恢复胰岛素敏感性。
