PDF(Sci-hub)
Abstract：
New ecological trends and changes in consumer behavior are known to favor biofilm formation in household appliances, increasing the need for new antimicrobial materials and surfaces. Their development requires laboratory-cultivated biofilms, or biofilm model systems (BMS), which allow for accelerated growth and offer better understanding of the underlying formation mechanisms. Here, we identified bacterial strains in wildtype biofilms from a variety of materials from domestic appliances using matrix-assisted laser desorption/ionization-time of flight mass spectroscopy (MALDI-TOF-MS). Staphylococci and pseudomonads were identified by MALDI-TOF-MS as the main genera in the habitats and were analyzed for biofilm formation using various in vitro methods. Standard quantitative biofilm assays were combined with scanning electron microscopy (SEM) to characterize biofilm formation. While Pseudomonas putida, a published lead germ, was not identified in any of the collected samples, Pseudomonas aeruginosa was found to be the most dominant biofilm producer. Water-born Pseudomonads were dominantly found in compartments with water contact only, such as in detergent compartment and detergent enemata. Furthermore, materials in contact with the washing load are predominantly colonized with bacteria from the human.
摘要：
众所周知，新的生态趋势和消费者行为的变化有利于家用电器中生物膜的形成，增加对新型抗微生物材料和表面的需求。它们的发展需要实验室培养的生物膜，或生物膜模型系统（BMS），这允许加速增长，并提供对潜在形成机制的更好理解。这里,我们使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）从家用电器的各种材料中鉴定了野生型生物膜中的细菌菌株。通过MALDI-TOF-MS将葡萄球菌和假单胞菌鉴定为栖息地的主要属，并使用各种体外方法分析了生物膜的形成。将标准定量生物膜测定与扫描电子显微镜(SEM)组合以表征生物膜形成。而恶臭假单胞菌,一种已发表的铅细菌，在任何收集的样本中都没有发现，铜绿假单胞菌被发现是最主要的生物膜生产者。在仅与水接触的隔室中主要发现了水出生的假单胞菌，如在洗涤剂室和洗涤剂灌肠。此外，与洗涤负载接触的材料主要由来自人类的细菌定殖。
