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Abstract：
A better understanding of their interaction with cell-based tissue is a fundamental prerequisite towards the safe production and application of engineered nanomaterials. Quantitative experimental data on the correlation between physicochemical characteristics and the interaction and transport of engineered nanomaterials across biological barriers, in particular, is still scarce, thus hampering the development of effective predictive non-testing strategies. Against this background, the presented study investigated the translocation of gold and silver nanoparticles across the gastrointestinal barrier along with related biological effects using an in vitro 3D-triple co-culture cell model. Standardized in vitro assays and quantitative polymerase chain reaction showed no significant influence of the applied nanoparticles on both cell viability and generation of reactive oxygen species. Transmission electron microscopy indicated an intact cell barrier during the translocation study. Single particle ICP-MS revealed a time-dependent increase of translocated nanoparticles independent of their size, shape, surface charge, and stability in cell culture medium. This quantitative data provided the experimental basis for the successful mathematical description of the nanoparticle transport kinetics using a non-linear mixed effects modeling approach. The results of this study may serve as a basis for the development of predictive tools for improved risk assessment of engineered nanomaterials in the future.
摘要：
更好地了解它们与基于细胞的组织的相互作用是工程纳米材料安全生产和应用的基本前提。关于物理化学特性与工程纳米材料跨生物屏障的相互作用和运输之间的相关性的定量实验数据，特别是,仍然稀缺，从而阻碍了有效的预测性非测试策略的开发。在这种背景下,本研究使用体外3D-三重共培养细胞模型研究了金和银纳米颗粒穿过胃肠道屏障的转运以及相关的生物学效应。标准化的体外测定和定量聚合酶链反应显示，所应用的纳米颗粒对细胞活力和活性氧的产生均无明显影响。透射电子显微镜显示在易位研究期间完整的细胞屏障。单粒子ICP-MS揭示了一个时间依赖性的增加易位的纳米粒子独立于它们的大小，形状,表面电荷,在细胞培养基中的稳定性。该定量数据为使用非线性混合效应建模方法成功地数学描述纳米颗粒传输动力学提供了实验基础。这项研究的结果可以作为未来开发预测工具的基础，以改善工程纳米材料的风险评估。
