Abstract:
Prunus zhengheensis is a novel species originated in Fujian province, China. However, there is no further information available on its classification and molecular biology study. In this study, we first report the complete chloroplast (cp) genome sequence of P. zhengheensis. The cp genome of P. zhengheensis is 158,106 bp and GC content is 36.73%, is a circular structure composed of LSC (large single copy), SSC (small single copy), and IR (inverted repeat) regions, with the size of the three regions being 86,321 bp, 18,999 bp and 26,393 bp, respectively. The cp genome of P. zhengheensis contains 130 genes, and 242 SSRs are identified in the cp genome. The comparative analysis of cp genomes in eight Prunus plants demonstrates the subtle divergences occur in the protein-coding gene rps18, rps12, psbF, rpl33, matK, and rbcL, and that the KA/KS nucleotide substitution ratio of the ndhF of P. zhengheensis and P. armeniaca is 1.79636. The phylogenetic results indicate that the P. zhengheensis is closely related to P. mume, compared to other species of Prunus. Our research results provide the important genomic information for molecular phylogeny of P. zhengheensis.
摘要:
李属是一种起源于福建省的新物种,中国。然而,没有关于其分类和分子生物学研究的进一步信息。在这项研究中,我们首先报道了振河松的完整叶绿体(cp)基因组序列。郑河松的cp基因组为158,106bp,GC含量为36.73%,是由LSC(大型单副本)组成的循环结构,SSC(小型单一副本),和IR(反向重复)区域,三个区域的大小为86,321bp,18,999个基点和26,393个基点,分别。郑河松的cp基因组包含130个基因,在cp基因组中鉴定出242个SSR。八株李属植物的cp基因组的比较分析表明,蛋白质编码基因rps18,rps12,psbF,rpl33,matK,和rbcL,振河假单胞菌和阿门虫的ndhF的KA/KS核苷酸取代比为1.79636。系统发育结果表明,郑河松与乌梅关系密切,与其他李属物种相比。我们的研究结果为振河松的分子系统发育提供了重要的基因组信息。