关键词: Active Ras-binding domain of Raf1 (RBD) Dictyostelium discoideum Fluorescence microscopy Macropinocytosis Phagocytosis Phosphatidylinositol 3,4,5-trisphosphate (PIP3) Phosphatidylinositol 4,5-bisphosphate (PIP2) Phosphoinositide 3-kinases (PI3K) Pleckstrin homology domain (PH domain) Ras

Mesh : Dictyostelium / physiology Enzyme Activation Gene Expression Regulation Humans Microscopy, Confocal Microscopy, Fluorescence Phagocytosis Phosphatidylinositol 3-Kinases / metabolism Pinocytosis Protozoan Proteins / metabolism Signal Transduction ras Proteins / metabolism

来  源:   DOI:10.1007/978-1-0716-1402-0_10

Abstract:
Macropinocytosis and phagocytosis are the processes by which eukaryotic cells use their plasma membrane to engulf liquid or a large particle and give rise to an internal compartment called the macropinosomes or phagosome, respectively. Dictyostelium discoideum provides a powerful system to understand the molecular mechanism of these two fundamental cellular processes that impact human health and disease. Recent developments in fluorescence microscopy allow direct visualization of intracellular signaling events with high temporal and spatial resolution. Here, we describe methods to visualize temporospatial activation or localization of key signaling components that are crucial for macropinocytosis and phagocytosis using confocal fluorescence microscopy.
摘要:
巨噬细胞吞噬和吞噬作用是真核细胞利用其质膜吞噬液体或大颗粒并产生称为巨噬细胞体或吞噬体的内部隔室的过程。分别。盘基网柄菌提供了一个强大的系统来了解影响人类健康和疾病的这两个基本细胞过程的分子机制。荧光显微镜的最新发展允许具有高时间和空间分辨率的细胞内信号事件的直接可视化。这里,我们描述了使用共聚焦荧光显微镜观察对大细胞胞吞和吞噬作用至关重要的关键信号成分的时空激活或定位的方法。
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