关键词： 16s rRNA AMR, anti microbial resistance AQ20, alignment quality Arcanobacterium haemolyticum CARD, the comprehensive antibiotic resistance database CDS, coding sequence Cellulitis Diabetic foot ulcer EUCAST, The European Committee on Antimicrobial Susceptibility Testing ISPs, ion sphere particles Ion PGM, ion personal genome machine MUSCLE, multiple sequence comparison by log- expectation NCBI, National Center for Biotechnology Information NDARO, national database of antibiotic resistant organisms Osteomyelitis PATRIC, the Pathosystems Resource Integration Center Q20, base call quality RASTtk, rapid annotation using subsystem technology tool kit RAxML, randomized axelerated maximum likelihood Whole-genome sequencing and annotation

来  源：   DOI：10.1016/j.idcr.2021.e01139   PDF(Sci-hub)   PDF(Pubmed)

Abstract：
Arcanobacterium haemolyticum can cause deep infections, including osteomyelitis. In this study, an automated system misidentified this causal agent as Cellulomonas species but 16 s rRNA sequencing correctly identified it as A. haemolyticum. Recognizing the capability of A. haemolyticum to establish the disease is of great importance to enable accurate diagnosis and begin the suitable antibiotic therapy. Here we present the first case of successfully treated A. haemolyticum infective osteomyelitis in a 64-year-old Saudi patient with diabetes mellitus type 2 and review the characteristics of this seldom pathogenic agent.
摘要：
溶血性弧菌可引起深部感染,包括骨髓炎.在这项研究中,一个自动化系统将这种病原体错误地鉴定为细胞单胞菌属，但16srRNA测序正确地将其鉴定为溶血杆菌。认识到溶血芽孢杆菌确定该疾病的能力对于能够准确诊断和开始合适的抗生素治疗非常重要。在这里，我们介绍了一名64岁的沙特阿拉伯2型糖尿病患者成功治疗的首例溶血性感染性骨髓炎，并回顾了这种很少致病因子的特征。
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