Abstract：
Myc is deregulated in most-if not all-cancers, and it not only promotes tumor progression by inducing cell proliferation but is also responsible for tumor immune evasion. In a nutshell, MYC promotes the development of tumor-associated macrophages, impairs the cellular response to interferons, induces the expression of immunosuppressive molecules, and excludes tumor infiltrating lymphocytes (TILs) from the tumor site. Based on the insights into the role of MYC in promoting and regulating immune evasion by cancer cells, it is of special interest to study the different immune cell populations infiltrating the tumors. MYC inhibition has emerged as a potential new strategy for the treatment of cancer, directly inhibiting tumor progression while also counteracting the immunosuppressive tumor microenvironment, allowing an optimal anti-tumor immune response. Hence, this chapter describes a flow cytometry-based method to study the different immune cell subsets infiltrating the tumor by combining surface, cytoplasmic, and nuclear multicolor protein stainings.
摘要：
Myc在大多数——如果不是所有——癌症中都被解除了管制，它不仅通过诱导细胞增殖促进肿瘤进展，而且还负责肿瘤免疫逃避。简而言之，MYC促进肿瘤相关巨噬细胞的发育，削弱细胞对干扰素的反应，诱导免疫抑制分子的表达，并排除肿瘤部位的肿瘤浸润淋巴细胞（TIL）。基于对MYC在促进和调节癌细胞免疫逃避中的作用的见解，研究浸润肿瘤的不同免疫细胞群具有特殊的意义。MYC抑制已成为治疗癌症的潜在新策略，直接抑制肿瘤进展，同时还抵消免疫抑制肿瘤微环境，允许最佳的抗肿瘤免疫反应。因此，本章介绍了一种基于流式细胞术的方法，通过结合表面研究浸润肿瘤的不同免疫细胞亚群，细胞质，和核多色蛋白染色。
