Abstract：
Diagnosis and prognosis of breast cancer is based on disease staging identified through histopathological and molecular biology techniques. Animal models are used to gain mechanistic insights into the development of breast cancer. C(3)1-TAg is a genetically engineered mouse model that develops mammary cancer. However, carcinogenesis caused by this transgene was characterized in the Friend Virus B (FVB) background. As most genetic studies are done in mice with C57BL/6 J background, we aimed to define the histological alterations in C3(1)-TAg C57BL/6 J animals. Our results showed that C3(1)-TAg animals with C57BL/6 J background develop solid-basaloid adenoid cystic carcinomas with increased fibrosis, decreased area of adipocytes, and a high proliferative index, which are triple-negative for progesterone, estrogen, and human epidermal growth factor receptor 2 (HER2) receptors. Our results also revealed that tumor development is slower in the C57BL/6 J background when compared with the FVB strain, providing a better model to study the different stages in breast cancer progression.
摘要：
乳腺癌的诊断和预后基于通过组织病理学和分子生物学技术鉴定的疾病分期。动物模型用于获得对乳腺癌发展的机制见解。C(3)1-TAG是发展乳腺癌的基因工程小鼠模型。然而,由这种转基因引起的致癌作用在朋友病毒B（FVB）背景下进行了表征。由于大多数遗传研究是在具有C57BL/6J背景的小鼠中进行的，我们旨在定义C3（1）-TAGC57BL/6J动物的组织学改变。我们的结果表明，C57BL/6J背景的C3（1）-TAG动物发展为纤维化增加的实性基底细胞样腺样囊性癌，脂肪细胞面积减少，和高增殖指数，孕酮是三阴性的，雌激素,和人表皮生长因子受体2（HER2）受体。我们的结果还表明，与FVB菌株相比，C57BL/6J背景下的肿瘤发展较慢，提供了一个更好的模型来研究乳腺癌进展的不同阶段。
