关键词: Antifungal agent Bioassay HPLC Polyene macrolide Streptomyces UV-visible spectrum

Mesh : Anti-Bacterial Agents / biosynthesis Antifungal Agents / metabolism Biological Assay / methods Chromatography, High Pressure Liquid / methods Fermentation / physiology Macrolides / metabolism Polyenes / metabolism Streptomyces / metabolism

来  源:   DOI:10.1007/978-1-0716-1358-0_19

Abstract:
Polyene antibiotics are macrolide antifungal compounds obtained by fermentation of producer Streptomyces strains. Here we describe commonly used methods for polyene production, detection, and their subsequent extraction and purification. While bioassays are used to detect these compounds based on their biological activity, quantification by spectrophotometry or high-performance liquid chromatography (HPLC ) relies on their physiochemical properties and is more reliable.
摘要:
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