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Abstract：
New psychoactive substances (NPS) are substances that continue to appear on the drug market to bypass controlled substance legislation. Mephedrone or 4-methylmethcathinone is becoming the most popular new psychoactive substance among youth as a recreational drug. The present study describes the optimization and validation of a sensitive method that combined clean up procedure and LC-MS/MS technique designed to simultaneously determine the presence of Mephedrone and its two metabolites (normephedrone as active metabolite and dyhidromephedrone) in post-mortem specimens (body fluids and organ tissues). To date, this is the first determination of Mephedrone metabolites in post-mortem specimens. The validated method was applied to a fatal Mephedrone intoxication case. The distribution of the three analytes in different post-mortem matrices was presented. The toxicological results of the studied case are discussed, along with autopsy, histopathological evidence and crime-scene information. The toxicological results presented in the study provide new data relative to mephedrone and the distribution of its metabolites in post-mortem specimens. In our opinion, the metabolite concentration database must be developed because the metabolites may be linked to toxicity. The pattern of parent drug and its metabolites can be helpful in the interpretation of fatal cases involving mephedrone, which will contribute to the currently limited knowledge about mephedrone and metabolites concentrations.
摘要：
新的精神活性物质（NPS）是继续出现在药物市场上以绕过受控物质立法的物质。Mepherone或4-甲基甲卡西酮正在成为年轻人中最受欢迎的新型精神活性物质作为休闲药物。本研究描述了一种敏感方法的优化和验证，该方法结合了清理程序和LC-MS/MS技术，旨在同时确定Mepherone及其两种代谢物（作为活性代谢物的normepherone和dyhidromepherone）的存在验尸标本（体液和器官组织）。迄今为止,这是在验尸标本中首次测定麦柏酮代谢物。经过验证的方法已应用于致命的美柏酮中毒病例。介绍了三种分析物在不同验尸基质中的分布。讨论了所研究病例的毒理学结果，随着尸检，组织病理学证据和犯罪现场信息.研究中提供的毒理学结果提供了有关甲氧麻黄酮及其代谢物在死后标本中分布的新数据。在我们看来,必须建立代谢物浓度数据库，因为代谢物可能与毒性有关。母体药物及其代谢物的模式有助于解释涉及甲氧麻黄酮的致命病例，这将有助于目前关于甲氧麻黄酮和代谢物浓度的有限知识。
