关键词： MMP1 STC1 chi-miR-8516 circ-140 mammary gland involution

Mesh ： Animals Caseins / genetics metabolism Cells, Cultured Epithelial Cells / metabolism Female Glycoproteins / genetics metabolism Goats Lactation / genetics Lipid Metabolism Mammary Glands, Animal / cytology metabolism Matrix Metalloproteinase 1 / genetics metabolism MicroRNAs / genetics metabolism STAT5 Transcription Factor / genetics metabolism TOR Serine-Threonine Kinases / genetics metabolism

来  源：   DOI：10.3390/genes12050671   PDF(Sci-hub)   PDF(Pubmed)

Abstract：
MicroRNAs play an essential role in mammary gland development, and involution is a factor that limits lactation. Chi-miR-8516 is one of the validated microRNAs that regulates the expression of STC1 and MMP1, which surge during the involution of the mammary gland. This study aims to explore the direct or indirect regulation of STC1 and MMP1 by chi-miR-8516 and the regulation of chi-miR-8516 by circ-140. In goat mammary epithelial cells, we found that chi-miR-8516 takes circ-140 as a sponge and regulates MMP1 expression by targeting STC1 and promoting the phosphorylation of MAPK. The examination of αs1-/β-casein and lipid showed the modulation of the circ-140/chi-miR-8516/STC1-MMP1 axis in casein secretion and lipid formation, which was regulated by the phosphorylation of mTOR and STAT5. This study illustrates an axis that regulates the synthesis of milk components, and explores the pathways in which the axis participates.
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