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Abstract：
In this study, Phellinus linteus polysaccharides (PLPS) and proteins were simultaneously separated from P. linteus mycelia by using an aqueous two-phase system (ATPS) based on choline chloride ([Chol]Cl)/K2HPO4, and the physicochemical and antioxidant properties of PLPS after ATPS extraction were evaluated. Results demonstrated that the maximal extraction efficiencies of 68.53% ± 0.29% PLPS and 82.37% ± 0.41% proteins were obtained when the cholinium-based ATPS contained 68.9% K2HPO4, 20% [Chol]Cl, 10.0 mg mL-1 crude water extract (1.0 mL), and distilled water (4.0 mL) at shaking time and temperature of 30 min and 21.2 °C, respectively. Compared with C-PLPS obtained using traditional ethanol precipitation and isolation protocols, PLPS had higher carbohydrate content (63.58% ± 1.12%), lower molecular weight (15.2 kDa, 80%), different monosaccharide compositions, and showed similar preliminary structural characterizations. Moreover, PLPS exhibited more evident scavenging effects on free radicals and in vitro antioxidant activities than C-PLPS. Therefore, the method of [Chol]Cl/K2HPO4 ATPS can be developed as an effective strategy for the separation/purification of highly bioactive polysaccharides.
摘要：
在这项研究中,采用基于氯化胆碱([Chol]Cl)/K2HPO4的双水相体系(ATPS),同时分离桑黄菌丝体中的桑黄多糖(PLPS)和蛋白质,并对ATPS提取后PLPS的理化和抗氧化性能进行评价。结果表明，当基于胆碱的ATPS含有68.9％K2HPO4，20％[Chol]Cl时，获得了68.53％±0.29％PLPS和82.37％±0.41％蛋白质的最大提取效率，10.0mgmL-1粗水提取物（1.0mL），和蒸馏水（4.0毫升），振荡时间和温度为30分钟和21.2°C，分别。与使用传统乙醇沉淀和分离方案获得的C-PLPS相比，PLPS的碳水化合物含量较高（63.58%±1.12%），较低分子量(15.2kDa，80%),不同的单糖组成，并显示出类似的初步结构特征。此外，PLPS比C-PLPS具有更明显的自由基清除作用和体外抗氧化活性。因此,[Chol]Cl/K2HPO4ATPS方法可作为分离/纯化高生物活性多糖的有效策略。
