Abstract：
The potential of naturally occurring substances as a source of biomedical materials is well-recognised and is being increasingly exploited. Silk fibroin membranes derived fromBombyx morisilk cocoons exemplify this, for example as substrata for the growth of ocular cells with the aim of generating biomaterial-cell constructs for tissue engineering. This study investigated the transport properties of selected silk fibroin membranes under conditions that allowed equilibrium hydration of the membranes to be maintained. The behaviour of natural fibroin membranes was compared with fibroin membranes that have been chemically modified with poly(ethylene glycol). The permeation of the smaller hydrated sodium ion was higher than that of the hydrated calcium ion for all three ethanol treated membranes investigated. The PEG and HRP-modified C membrane, which had the highest water content at 59.6 ± 1.5% exhibited the highest permeation of the three membranes at 95.7 ± 2.8 × 10-8cm2s-1compared with 17.9 ± 0.9 × 10-8cm2s-1and 8.7 ± 1.7 × 10-8cm2s-1for membranes A and B respectively for the NaCl permeant. Poly(ethylene glycol) was used to increase permeability while exploiting the crosslinking capabilities of horseradish peroxidase to increase the compressive strength of the membrane. Importantly, we have established that the permeation behaviour of water-soluble permeants with hydrated radii in the sub-nanometer range is analogous to that of conventional hydrogel polymers.
摘要：
天然存在的物质作为生物医学材料来源的潜力已得到充分认可，并且正被越来越多地利用。来自BombyxMorisilk茧的丝素蛋白膜证明了这一点，例如作为眼细胞生长的基质，目的是产生用于组织工程的生物材料-细胞构建体。这项研究调查了在允许维持膜的平衡水合的条件下选择的丝素蛋白膜的运输特性。将天然丝素蛋白膜的行为与已用聚（乙二醇）化学修饰的丝素蛋白膜进行了比较。对于所研究的所有三种乙醇处理的膜，较小的水合钠离子的渗透均高于水合钙离子的渗透。PEG和HRP修饰的C膜，水含量最高，为59.6±1.5％，三种膜的渗透率最高，为95.7±2.8×10-8cm2s-1，而膜A和膜B的渗透率分别为17.9±0.9×10-8cm2s-1和8.7±1.7×10-8cm2s-1。聚（乙二醇）用于增加渗透性，同时利用辣根过氧化物酶的交联能力来增加膜的抗压强度。重要的是,我们已经确定，水合半径在亚纳米范围内的水溶性渗透物的渗透行为类似于常规水凝胶聚合物的渗透行为。
