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Abstract：
Potato (Solanum tuberosum L.) is an important staple food and economic crop in many countries. It is of critical importance to understand the genetic diversity and population structure for effective collection, conservation, and utilization of potato germplasm. Thus, the objective of the present study was to investigate the genetic diversity and population structure of potato germplasm conserved in the National Agrobiodiversity Center (NAC) of South Korea to provide basic data for future preservation and breeding of potato genetic resources. A total of 24 simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure of 482 potato accessions. A total of 257 alleles were detected, with an average of 10.71 alleles per locus. Analysis of molecular variance showed that 97% of allelic diversity was attributed to individual accessions within the population, while only 3% was distributed among populations. Results of genetic structure analysis based on STRUCTURE and discriminant analysis of principal components revealed that 482 potato accessions could be divided into two main subpopulations. Accessions of subpopulation 1 mainly belonged to cultivars and breeding lines. Accessions of subpopulations 2 basically corresponded to wild relatives of potatoes. Results of this study provide useful information for potato improvement and conservation programs, although further studies are needed for a more accurate evaluation of genetic diversity and phenotypic traits of potatoes.
摘要：
马铃薯（SolanumtuberosumL.）是许多国家的重要主食和经济作物。了解遗传多样性和种群结构对于有效收集至关重要，养护,和马铃薯种质资源的利用。因此，本研究的目的是调查韩国国家农业生物多样性中心（NAC）保存的马铃薯种质的遗传多样性和种群结构，为将来马铃薯遗传资源的保存和育种提供基础数据。总共使用24个简单序列重复（SSR）标记来评估482个马铃薯种质的遗传多样性和种群结构。共检测到257个等位基因，每个基因座平均有10.71个等位基因。分子方差分析表明，97%的等位基因多样性归因于种群内的个体种质，而只有3%分布在人群中。基于结构和主成分判别分析的遗传结构分析结果表明，482个马铃薯种质可分为两个主要亚群。亚群1的加入主要属于品种和育种系。亚群2的加入量基本上对应于马铃薯的野生亲戚。这项研究的结果为马铃薯的改良和保护计划提供了有用的信息，尽管需要进一步研究才能更准确地评估马铃薯的遗传多样性和表型性状。
