PDF(Pubmed)
Abstract：
The Pacific white shrimp (Litopenaeus vannamei) is the most widely cultured shrimp in the world. A great attention has been paid to improve its body weight (BW) at harvest through genetic selection for decades. Genome-wide association study (GWAS) is a tool to dissect the genetic basis of the traits. In this study, a GWAS approach was conducted to find genes related to BW through genotyping 94,113 single nucleotide polymorphisms (SNPs) in 200 individuals from a breeding population. Four BW-related SNPs located in LG19 and LG39 were identified. Through further candidate gene association analysis, the SNPs in two candidate genes, deoxycytidylate deaminase and non-receptor protein tyrosine kinase, were found to be related with the body weight of the shrimp. Marker-assisted best linear unbiased prediction (MA-BLUP) based on the SNPs in these two genes was used to estimate the breeding values, and the result showed that the highest prediction accuracy of MA-BLUP was increased by 9.4% than traditional BLUP. These results will provide useful information for the marker-assisted breeding in L. vannamei.
摘要：
太平洋白虾（凡纳滨对虾）是世界上养殖最广泛的虾。几十年来，人们一直非常重视通过遗传选择在收获时改善其体重（BW）。全基因组关联研究（GWAS）是剖析性状遗传基础的工具。在这项研究中,进行GWAS方法,通过对200个育种群体个体的94,113个单核苷酸多态性(SNPs)进行基因分型,寻找与BW相关的基因.鉴定了位于LG19和LG39中的四个BW相关SNP。通过进一步的候选基因关联分析,两个候选基因中的SNP，脱氧胞苷酸脱氨酶和非受体蛋白酪氨酸激酶，被发现与虾的体重有关。标记辅助的最佳线性无偏预测（MA-BLUP）基于这两个基因的SNP被用来估计育种值，结果表明，MA-BLUP的最高预测精度比传统BLUP提高了9.4％。这些结果将为凡纳滨对虾的标记辅助育种提供有用的信息。
