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Abstract：
A novel functional drink with nutraceutical properties was formulated from the aqueous extracts of Ilex guayusa, and Vernonanthura patens leaves, and cocoa husks. This juice contains various bioactive compounds, such as phenolic compounds and methylxanthines, with antioxidant and stimulant properties of pharmacological interest. However, it is known whether herbal extracts\' interaction may have adverse toxic effects on human health. To evaluate this functional drink\'s innocuity, we estimated the acute oral toxicity (AOT) in experimental mice. This paper presents the AOT evaluation of two formulations of a functional drink (pre-formulation and microencapsulation) at a single dose of 2000 mg/kg of body weight (b.w.). No signs of adverse toxicity and mortality were observed after a single oral dose of 2000 mg/kg b.w. Likewise, no significant body and organ weight changes, food and water consumption behavior, and no histopathological changes were observed in the main organs evaluated. In conclusion, this functional drink can be categorized as low toxicity \" according to the Globally Harmonized Classification System (GHS), making it a potential beverage with high nutritional and pharmacological value.
摘要：
从Ilexguayusa的水提取物中配制了一种具有营养保健特性的新型功能饮料，和Vernonanthurapatens的叶子，还有可可皮.这种果汁含有各种生物活性化合物，如酚类化合物和甲基黄嘌呤，具有药理意义的抗氧化和兴奋剂特性。然而,已知草药提取物的相互作用是否会对人体健康产生不利的毒性影响。为了评价这种功能性饮料的无毒性,我们估计了实验小鼠的急性口服毒性（AOT）。本文介绍了2000mg/kg体重（b.w.）单剂量的功能性饮料的两种配方（预配方和微囊化）的AOT评估。在单次口服剂量为2000mg/kg体重后，没有观察到不良毒性和死亡率的迹象。同样，没有明显的身体和器官重量变化，食物和水的消费行为，在评估的主要器官中未观察到组织病理学变化。总之,根据全球统一分类系统（GHS），这种功能性饮料可以归类为低毒性，使其成为具有高营养和药理价值的潜在饮料。
