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Abstract：
This study is evaluating the effects of ethanol and nicotine exposure during pregnancy and lactation on placenta histology and follicular atresia in the first-generation (f1) mice pups. The experimental groups were 5 groups of NMRI pregnant mice, including: control, vehicle (received normal saline) ethanol (3 g/kg/day, 20 % v/v intraperitoneally), nicotine (1 mg/kg/day, subcutaneously), and ethanol plus nicotine which received both. Pregnant animals in each group were then divided into two groups, one group for examining the placenta that was treated for 18 days and the other group for the ovary of one-day-old (PND1) and fifty-six-day-old (PND56) female offspring who were treated for 42 days (during intrauterine development and lactation). After the autopsy procedure, histopathological and morphometrical observations were done. Data revealed that the exposed mice had a significant change in the placenta morphometry and histology as well as a marked increase in the number of ovarian TUNEL positive cells on postnatal days 1 and 56. Therefore, maternal exposure to alcohol and nicotine during developmental and lactation periods could lead to changes in the placenta properties as well as an increase in the apoptotic ovarian follicles in f1 mice pups.
摘要：
这项研究正在评估怀孕和哺乳期乙醇和尼古丁暴露对第一代（f1）小鼠幼崽胎盘组织学和卵泡闭锁的影响。实验组为5组NMRI孕鼠,包括:控制,溶媒（接受生理盐水）乙醇（3g/kg/天，20%v/v腹膜内)，尼古丁（1毫克/千克/天，皮下)，和乙醇加尼古丁两者兼得。然后将每组怀孕的动物分成两组,一组用于检查接受18天治疗的胎盘，另一组用于接受42天治疗的1日龄（PND1）和56日龄（PND56）雌性后代的卵巢（在宫内发育和泌乳期间）。验尸程序后,进行了组织病理学和形态计量学观察。数据显示，在出生后第1天和第56天，暴露的小鼠的胎盘形态和组织学发生了显着变化，并且卵巢TUNEL阳性细胞的数量显着增加。因此,母体在发育期和哺乳期暴露于酒精和尼古丁可能导致f1小鼠幼崽胎盘特性的变化以及凋亡卵泡的增加。
